A medium for the enumeration of coliform organisms in food and dairy products according to ISO 4832:2006 and is not intended for use in the diagnosis of disease or other conditions in humans. The selectivity of the medium is due to the presence of bile salts and crystal violet. Lactose fermenters produce red or purple colonies, often surrounded by a halo of the same color. Non-lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42–44 °C.

Typical Formulation

Formula	Litre
Yeast Extract	3.0 g/L
Enzymatic Digest of Animal Tissue	7.0 g/L
Sodium Chloride	5.0 g/L
Bile Salts	1.5 g/L
Lactose	10.0 g/L
Neutral Red	0.03 g/L
Crystal Violet	0.002 g/L
Agar	12.0 g/L

Preparation

Suspend 38.5g of the medium in one liter of purified water.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
DO NOT AUTOCLAVE.
Cool to 45–50 °C.

Previous Item Number: Product was previously known as LabM Product: LAB031 Violet Red Bile Agar (VRBA)

Technical Specification Sheet

Safety Data Sheet

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