MRS Agar, 500g
R 1,094.59
- Brand: Neogen® Culture Media
- Analyte: Lactobacillus spp.
- Platform: Dehydrated Culture Media
- Mass: 500 g
- Grams per Litre: 70.00
- Litres per 500 g: 7.00
- Plates per 500 g: 390.00
- No supplements required
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Availability:In stock
SKU
MNCM0190
BrandNeogen
MRS Agar is a medium for the enumeration of mesophilic lactic acid bacteria according to ISO 15214:1998, and is not intended for use in the diagnosis of disease or other conditions in humans.
MRS Agar was originally developed in 1960 by de Man, Rogosa & Sharpe for the cultivation and enumeration of Lactobacillus spp. from various sources and is intended as a substitute for Tomato Juice Agar. This original formulation has been adapted and adjusted to pH 5.7 according to ISO 15214:1998.
Nutrition is provided by enzymatic digest of casein, glucose, meat & yeast extracts whilst polyoxyethylenesorbitan monooleate, magnesium and manganese sulphates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli whilst inhibiting a number of other organism groups.
Occasionally, sterilization of this medium at 121°C can cause the pH to fall outside of the specified pH limits of 5.7 +/- 0.1. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
Typical Formulation
| Formula | Liter |
| Enzymatic Digest of Casein | 10.0 g/L |
| Meat Extract | 10.0 g/L |
| Yeast Extract | 4.0 g/L |
| Triammonium Citrate | 2.0 g/L |
| Sodium Acetate | 5.0 g/L |
| Magnesium Sulphate Heptahydrate | 0.2 g/L |
| Manganese Sulphate Tetrahydrate | 0.05 g/L |
| Dipotassium Hydrogen Phosphate | 2.0 g/L |
| Glucose | 20.0 g/L |
| Polyoxyethylenesorbitan Monooleate | 1.08 g/L |
| Agar | 15.5 g/L |
Preparation
- Suspend 70 grams of the medium in one litre of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121 ºC for 15 minutes.
- Cool to 45–50 ºC.
Previous Item Number: Product was previously known as LabM Product: LAB223 MRS Agar (ISO)
| Brand | Neogen |
|---|
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