Don’t waste your precious time any longer - revolutionise your protein research now with the Bio-Rad V3 Western Workflow. Bio-Rad’s V3 Workflow is a complete Western Blot workflow which saves you time, increases your accuracy and enables you to easily identify pain points.
Bio-Rad’s Western Blotting workflow is a seamless integration of products designed to work together to offer guaranteed results.
This workflow allows you to quickly check electrophoresis and blot transfer quality, obtaining truly quantitative results in just 5 simple steps:
- Separate proteins — Bio-Rad’s vertical electrophoresis systems offer superior protein separation with fast run times while making use of standard running buffers. With TGX Stain-Free Precast Gels, a unique gel chemistry technology, high-quality protein separation takes place in as little as 15 minutes.
- Visualize proteins — Confirm successful separation using stain-free technology. With just a 1-minute activation step on a Chemidoc stain-free enabled imager, you can immediately visualize proteins across the whole gel without ever staining.
- Transfer proteins — Rapidly and efficiently transfer proteins across a wide range of molecular weights. The Transblot turbo offers fast protein transfer, achieved in just 3 minutes.
- Verify protein transfer — The Chemidoc stain-free enabled Imagers allow for instant verification of protein transfer without the need for separate Ponceau staining. Next, immunodetect your protein of interest using Bio-Rad antibodies coupled with Clarity ECL substrates.
- Validate and quantitate —Perform multiplex or chemiluminescent blot detection using the Bio-Rad Chemidoc MP. Image Lab software allows you to validate results using total protein normalization. Normalizing to total protein makes stripping and re-probing a thing of the past.
Revolutionize your western blotting protocol with the next big thing in protein research, the Bio-Rad V3 Western Workflow.
Visit analytica Lab Africa and talk to Lasec® and Bio-Rad about changing the way you western blot.
- Luke Forbes, Application Specialist - Molecular Biology