Total RNA Purification Kits, 2 x 96-Well Plates
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- Format: High-Throughput
- Size: 2 x 96-well plates
- Total RNA Purification 96-Well Kit for the rapid purification of total RNA, including microRNA, without the use of phenol
- Fast and easy processing using rapid spin-column format
- Isolate total RNA, from large rRNA down to microRNA (miRNA)
- No phenol or chloroform extractions
- Isolate high-quality total RNA from a variety of sources
- RNA can be isolated and detected from as little as a single animal cell
- For research use only and NOT intended for in vitro diagnostics.
Optional accessories are available for purchase separately. If you require a customised solution, please contact our technical specialists by selecting the "Add to enquiry" button and using our enquiry form or at sales@lasec.com.
Installation, training and special delivery requirements, such as the need for tail-lift trucks and additional labour and installation for large equipment and hazardous material (Hazmat) packaging, are chargeable in addition to standard freight and will be quoted separately before dispatch.
Online ordering is currently limited to South African customers, but our full range is still available. If you are ordering from outside South Africa, please log an enquiry or email internationallabsales@lasec.com, and our international sales team will gladly assist you through our traditional sales channels.
Availability:On Request
SKU
BNOR24300
BrandNorgen Biotek Corp.
Norgen’s Total RNA Purification Kits 96-Well provide a rapid method for the high-throughput isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast, fungi, and plants. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components, such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications, including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Advantages of the Total RNA Purification Kits, 96-Well:
- Extract high-quality and quantity total RNA, including miRNA
- No phenol step required; isolate all RNA in one fraction
- Bind and elute all RNA, irrespective of size or GC content, without bias
- Very sensitive and linear down to a few cells without the need for carrier RNA
- Isolate from a wide variety of specimens
- Purified RNA is suitable for a variety of downstream applications, including small-RNA sequencing.
- Available in a variety of formats to suit your needs
- Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
Total RNA Purification Kits Components, 2 x 96-Well Plates:
- Buffer RL: 2 x 40 mL
- Wash Solution A: 2 x 38 mL
- Elution Solution A: 2 x 20 mL
- 96-Well Isolation Plate: 2
- Adhesive Tape: 4
- 96-Well Collection Plate: 2
- 96-Well Elution Plate: 2
- Product Insert: 1
| Kit Specifications | |
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material | |
| Animal Cells | 1 x 106 cells |
| Animal Tissues | 10 mg |
| Blood | 100 μL |
| Plasma/Serum | 150 μL |
| Bacteria | 1 x 109 cells |
| Yeast | 1 x 108 cells |
| Fungi | 40 mg |
| Plant Tissues | 40 mg |
| Time to Complete 96 Purifications | 30 minutes |
| Average Yields* | |
| HeLa Cells (1 x 106 cells) | 15 μg |
| E. coli (1 x 109 cells) | 50 μg |
* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
| Brand | Norgen Biotek Corp. |
|---|
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