The ReadyPrep protein extraction kit (total protein) provides a simple, rapid, and reproducible method for preparation of total cellular protein extracts from a wide variety of biological samples. Use of this kit generates protein samples that can be applied directly to a variety of applications, including IEF and 2-D gel separation.
The ReadyPrep protein extraction kit (total protein):
Includes a strongly chaotropic extraction solution containing the zwitterionic detergent ASB-14
Provides reagents for up to 20 extractions, each using 50–100 mg of cells or tissue
Can easily be scaled up to accommodate larger amounts of cells or tissue
Generates total protein samples in about 45 minutes