Name: Operetta CLS High-Content Analysis System
Brand: Revvity
SKU: IREVHH16000020
Availability: OutOfStock

Details

Product Overview

The Operetta CLS is a high-content analysis system combining confocal spinning-disk and widefield fluorescence imaging with brightfield and digital phase contrast modes. Three configurations span from a four-LED sensitivity-focused system to a fully equipped live-cell platform with water-immersion objectives and environmental control. Powered by Harmony software, the system guides users from image acquisition through to analysis and evaluation without requiring specialist image analysis expertise. PhenoLOGIC machine-learning technology enables custom algorithm creation, and the system integrates with Revvity's broader HCS workflow, including PhenoPlate microplates, PhenoVue reagents, Image Artist, and Signals One.

Key Technology Features

Frame rate: Up to 105 fps
Camera: sCMOS large format
LEDs (max): 8 excitation sources
Imaging modalities: Widefield, Confocal, BF, DPC
Dimensions (W x D x H): 66 x 98 x 45 cm
Automation: Compatible

Fluorescent Channel Specifications

All configurations use an sCMOS camera, include transmitted light, and are robotics/automation compatible. Quattro and FLEX configurations are field-upgradeable.

	Quattro For common applications requiring sensitivity and resolution, with capacity to grow	FLEX Flexible excitation and imaging modes for challenging applications; upgradeable to higher performance	LIVE Optimised for gentle, highly sensitive live-cell imaging
LEDs	4	8	8
Confocal disk	Optional	Included	Included
Environmental control	Optional	Optional	Included
Water immersion	Optional	Optional	Included
Confocal fluorescence	Optional	Included	Included
3D imaging	Optional	Included	Included
Live-cell imaging	Optional	Optional	Included
Widefield fluorescence	Included	Included	Included
BF and DPC	Included	Included	Included
FRET	Included	Included	Included

BF = brightfield. DPC = digital phase contrast. All configurations include robotics/automation compatibility and transmitted light. sCMOS camera is standard across all configurations.

Instrument Capabilities

Confocal Spinning-Disk Technology: Spinning-disk confocal optics provide efficient background rejection and gentle illumination, particularly important for live-cell experiments where phototoxicity and photobleaching need to be minimised. Synchronized LED illumination provides stable excitation.

Automated Water-Immersion Objectives: Available on FLEX and LIVE configurations. Water-immersion objectives deliver faster exposure times, increased resolution, and reduced photodamage compared with air objectives backed by more than 20 years of development experience.

High-Efficiency Excitation Design: Direct coupling of the excitation path limits light loss associated with light guides or fibre delivery, maximising the illumination reaching the sample and supporting efficient detection of low-signal fluorescent targets.

Fast Frame Rate Imaging: An imaging frame rate of up to 105 fps supports capture of rapid cellular events and dynamic processes, enabling kinetic and time-lapse studies without missing transient changes.

Intelligent Image Acquisition: Targeted acquisition focuses on objects of interest to reduce acquisition and analysis times. Particularly useful for 3D microtissue imaging and rare event studies where whole-well scanning would be inefficient.

3D Model Analysis: The large-format sCMOS camera and water-immersion objectives provide sensitivity and resolution for complex 3D models. The XYZ viewer and 3D viewer in Harmony software enable volumetric phenotypic readouts from spheroids and microtissues.

Application Areas

Fixed-cell assays

Widefield fluorescence
Spinning-disk confocal
Up to 8 excitation sources
User-accessible emission filters

Complex cellular models

3D microtissue imaging
Spheroid analysis
Volumetric phenotypic readouts
Rare event detection

Advanced assays

FRET
Ratiometric imaging
Protein-protein interactions
Conformational change analysis

Phenotypic fingerprinting

Subtle phenotypic change detection
Texture morphology analysis
STAR morphology analysis
Machine learning classification

Live-cell assays

Spinning-disk confocal
Brightfield
Digital phase contrast
Low phototoxicity
Environmental control (LIVE)

Harmony Software

Harmony high-content imaging and analysis software is included with the Operetta CLS system. It is designed for biologists to operate independently, without requiring specialist image analysis expertise.

Intuitive workflow: Guides users from image acquisition through analysis and evaluation in a structured, step-by-step interface.

Templates for quick setup: Pre-built acquisition templates let users configure channels and parameters efficiently without building from scratch.

Ready-made analysis solutions: Pre-built solutions cover common image analysis tasks, reducing setup time for standard assays.

Customisable building blocks: Users can create and configure their own high-content analysis applications using modular image analysis building blocks.

PhenoLOGIC machine learning: Proprietary machine-learning technology allows custom algorithm creation without image analysis expertise. Identifies subtle phenotypic differences.

Advanced morphology analysis: Includes texture and STAR morphology analysis for detailed cellular phenotype characterisation and robust phenotype differentiation.

Data management: Automatically stores analysis results and metadata, including assay layout, instrument settings, and user-defined annotations.

3D and XYZ visualisation: Dedicated 3D and XYZ viewers enable exploration and quantification of volumetric phenotypic readouts from complex 3D models.

HCS Ecosystem Integration

PhenoPlate microplates for HCS
PhenoVue cellular imaging reagents and cell painting kits
Image Artist image analysis and management platform
Signals One multivariate statistical analysis
Automation and robotic integration
Revvity automated cellular and drug discovery workflows

Image Artist and Signals One are sold separately and are not included with the Operetta CLS system.

Technical Specifications

Dimensions (W x D x H)	66.0 cm x 98.0 cm x 45.0 cm
Camera	Large-format sCMOS
Frame rate	Up to 105 fps
LEDs	4 (Quattro) or 8 (FLEX, LIVE)
Imaging modalities	Widefield fluorescence, confocal fluorescence, brightfield, digital phase contrast, FRET
Automation compatible	Yes (all configurations)
Unit size	1 unit

For research use only. Not for use in diagnostic procedures. Image Artist and Signals One are sold separately. PhenoPlate, PhenoVue, PhenoLOGIC, and Harmony are trademarks of Revvity.

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