Opera Phenix OptIQ High-Content Screening System
Enquire for Price
- Configurations: Single (1 camera), Simultaneous (2 cameras), FRET (4 cameras, CFP/YFP), Screener (4 cameras, 4 high-power lasers)
- Imaging modalities: Confocal fluorescence, widefield fluorescence, brightfield, digital phase contrast, ratiometric FRET
- Confocal technology: Microlens-enhanced Nipkow spinning disk with Synchrony Optics for minimal spectral crosstalk
- Cameras: Up to 4 confocal cameras, greater than 95% quantum efficiency
- Frame rate: Up to 100 fps
- Lasers: 375/425, 405, 488, 561, 640 nm (configuration dependent)
- Objectives: Custom high-NA water-immersion (all configurations)
- Environmental control: Included (Single, FRET) / optional (Simultaneous, Screener)
- On-board liquid handling: Optional (Simultaneous, FRET, Screener)
- Key applications: 3D organoid and organ-on-chip imaging, functional genomic screening, drug sensitivity testing, cell painting, large library screening, FRET, hypoxia studies
- Software: Harmony with Phenologic.AI deep-learning classification and Find Organoids building block
- Automation compatible: Yes (all configurations)
For research use only. Not for use in diagnostic procedures.
Need a customised solution?
Optional accessories are available for purchase separately. If your order needs a customised configuration, our technical specialists will follow up with full details before your order is finalised. Contact us via the enquiry form or sales@lasec.com.
Installation, training and special delivery
Some products require installation, training, or special delivery (such as tail-lift trucks, extra labour, or hazardous material packaging). If your order needs this, we'll contact you with the additional cost before dispatch, so your final invoice may differ from the listed price.
Ordering from outside South Africa
Online ordering is currently limited to South African customers, but our full range is still available. Log an enquiry or email international@lasec.com and our international sales team will assist you through our traditional sales channels.
DISCLAIMER: All product images are for illustrative purposes only. Actual products may vary slightly in colours, controls, and visible features.
Availability:On Request
SKU
IREVOPPHEOPT
BrandRevvity
Product Overview
The Opera Phenix OptIQ is a high-content screening system built for complex, high-throughput applications, including phenotypic screening, 3D organoid analysis, organ-on-chip imaging, functional genomic screening, and drug sensitivity testing. Four configurations scale from a single-camera system with upgrade potential through to a four-camera, four-laser screener for large library workflows. The microlens-enhanced spinning disk confocal design, proprietary Synchrony Optics for reduced spectral crosstalk, custom high-NA water-immersion objectives, and up to 100 fps frame rate combine to deliver sensitivity and resolution in thick and 3D
What is New in the OptIQ
- >95% QE cameras
- Advanced laser-based autofocus
- Hypoxia applications
- 10x high NA lens
- AI classification tools
- Find Organoids building block
Key Technology Features
- Max cameras: 4 confocal
- Camera QE: >95 %
- Frame rate: Up to 100 fps
- Confocal type: Microlens spinning disk
- Optics: Synchrony Optics
- Automation: Compatible
Instrument Capabilities
Microlens-Enhanced Spinning Disk Confocality: A pinhole and microlens disk spin in tandem for increased light efficiency. Pinhole distances are optimised for 3D samples, reducing out-of-focus light and improving signal quality in thick specimens such as organoids and spheroids.
Proprietary Synchrony Optics: Dual-view excitation of neighbouring spectral channels separates adjacent fluorescence excitation and emission patterns, minimising spectral crosstalk during simultaneous multi-colour acquisition. Delivers greater speed and higher sensitivity.
High-QE Multi-Camera System: Up to four cameras with greater than 95% quantum efficiency support simultaneous multi-colour confocal acquisition, significantly increasing imaging speed for extensive z-stacks and 3D models. Each additional camera increases throughput without sacrificing sensitivity.
Water-Immersion Objectives: Custom-designed high-NA water-immersion objectives capture more photons, improve z-resolution, and maintain image quality in thick samples. Automated objective switching is supported across all configurations.
Intelligent Image Acquisition and PreciScan: Targeted acquisition images only objects of interest, centred 3D models at high magnification, reducing imaging time and data volume. PreciScan enables pre-scan and re-scan workflows for efficient rare event or high-content studies.
Hypoxic Environment Imaging: The onboard environmental control chamber allows cells to be imaged at low oxygen concentrations, enabling more physiologically relevant experimental conditions for hypoxia studies.
Find Organoids Building Block: A dedicated building block within Harmony software detects organoids in brightfield stacks using 2D or 3D detection, with improved object splitting, streamlining analysis setup for organoid-based assays.
On-Board Liquid Handling (Optional): An optional on-board pipettor module supports kinetic assays by enabling fast-response capture of cellular events triggered by reagent addition during imaging available on Simultaneous, FRET, and Screener configurations.
Configurations
All four configurations include water-immersion lenses, transmitted-light capability, robotics compatibility, and the full suite of imaging modes, except where noted. The Single configuration is upgradeable with additional cameras.
| Single | Simultaneous | FRET | Screener | |
|---|---|---|---|---|
| Cameras and lasers | ||||
| Confocal cameras | 1 | 2 | 4 | 4 |
| 375/425 nm laser | — | — | Yes | — |
| 405 nm laser | Yes | Yes | — | Yes |
| 488 nm laser | Yes | Yes | Yes | Yes |
| 561 nm laser | Yes | Yes | Yes | Yes |
| 640 nm laser | Yes | Yes | Yes | Yes |
| System options | ||||
| Environmental control | Included | Optional | Included | Optional |
| Water immersion lenses | Included | Included | Included | Included |
| On-board liquid handling | — | Optional | Optional | Optional |
| Robotics / automation | Yes | Yes | Yes | Yes |
| Imaging modes | ||||
| Widefield fluorescence | Yes | Yes | Yes | Yes |
| Multi-colour confocal | Yes | Yes | Yes | Yes |
| Simultaneous multi-colour confocal | — | Yes | Yes | Yes |
| Brightfield and DPC | Yes | Yes | Yes | Yes |
| 3D imaging | Yes | Yes | Yes | Yes |
| Ratiometric FRET | Suitable | Suitable | CFP/YFP | Suitable |
| Fast frame rate imaging | Yes | Yes | Yes | Yes |
| PreciScan (pre/re-scan) | Yes | Yes | Yes | Yes |
| Minimal crosstalk imaging | Yes | Yes | Yes | Yes |
DPC = digital phase contrast. The Single configuration is upgradeable with additional cameras. The FRET configuration is optimised for CFP/YFP FRET applications.
Application Areas
Drug discovery and screening
- Drug sensitivity testing
- Large library screening (Screener)
- Cell painting
- Multiparametric screening
Advanced fluorescence
- CFP/YFP FRET (FRET configuration)
- Ratiometric imaging
- Protein-protein interactions
- Phenotypic fingerprinting
Functional genomic screening
- Phenotypic response to gene manipulation
- High-throughput genetic evidence generation
Environmental and physiological studies
- Hypoxia applications
- Live-cell imaging with environmental control
Microphysiological systems and 3D models
- Organoid imaging and analysis
- Organ-on-chip
- Spheroid growth tracking
- 3D invasion into Matrigel
- Volumetric phenotypic readouts
Live-cell assays
- Rapid cellular event capture
- On-board reagent dispensing
- Dynamic process monitoring
Harmony Software and Phenologic.AI
Intuitive workflow: Guides users from image acquisition through analysis and evaluation in a structured interface designed for biologists to operate independently.
Templates and ready-made solutions: Pre-built acquisition templates and analysis solutions reduce setup time for common assays and acquisition channel configuration.
Customisable building blocks: Modular image analysis building blocks allow users to create and configure custom high-content analysis applications without coding.
Find Organoids building block: Detects organoids in brightfield stacks using 2D or 3D detection with improved object splitting, streamlining organoid-based assay analysis.
Phenologic.AI: Pre-trained deep learning models enable AI-based segmentation and classification of cells in brightfield and fluorescent images for live- and fixed-cell assays — without requiring machine learning expertise.
Advanced morphology analysis: Texture and STAR morphology analysis tools provide detailed cellular phenotype characterisation and robust differentiation of subtle phenotypic differences.
3D and XYZ visualisation: Dedicated 3D and XYZ viewers enable exploration and quantification of volumetric phenotypic readouts from organoids, spheroids, and other 3D models.
Data management: Automatically stores analysis results and metadata, including assay layout, instrument settings, and user-defined annotations.
| Imaging modalities | Confocal fluorescence, widefield fluorescence, brightfield, digital phase contrast, FRET |
| Max cameras (confocal) | 4 |
| Camera QE | >95% |
| Frame rate | Up to 100 fps |
| Confocal technology | Microlens-enhanced Nipkow spinning disk |
| Optics | Synchrony Optics (dual-view excitation, minimal crosstalk) |
| Objectives | Custom high-NA water-immersion (all configurations) |
| Automation compatible | Yes (all configurations) |
| Software | Harmony; PhenoLogic.AI |
| Unit size | 1 unit |
For research use only. Not for use in diagnostic procedures. Synchrony Optics, PhenoLOGIC, Phenologic.AI, Harmony, PhenoPlate, and PreciScan are trademarks of Revvity. AlexaFluor is a trademark of Life Technologies.
| Brand | Revvity |
|---|
Write Your Own Review
Related Products By Brand
Enquire
Enquire
Enquire
Enquire
Enquire