Cellometer K2 Fluorescent Cell Counter
Enquire for Price
- Imaging modality: Dual fluorescence (AO/PI) and brightfield
- Primary applications: Hepatocytes, stem cells, splenocytes, tumour suspensions, whole blood, peripheral blood, cord blood, bone marrow, primary cells
- Results in: Under 60 seconds
- Minimum sample volume: 10 µl
- Cell size range: 4–90 µm
- Concentration range: 10⁵ – 10⁷ cells/ml
- Preloaded cell types: Over 400
- Images per sample: 1, 4, or 8 (default: 4)
- Predefined assays: Viability, apoptosis, transfection efficiency
- Fluorescence optics modules included: 470/535 nm (AO, CFDA, FITC) and 540/660 nm (PI, Ethidium Bromide, 7-AAD)
- Report export formats: CSV, Excel, Word, PDF
- Dimensions (W x D x H): 15.2 cm x 21.6 cm x 35.6 cm
- Weight: 10.4 kg
- Power supply: 100–240 V AC, 50/60 Hz
- Connectivity: USB 2.0
- 21 CFR Part 11: Optional module available
- PC laptop: Optional accessory (Windows 11, 64-bit; Intel Core i7; 16 GB RAM; NVIDIA P1000 GPU required)
For research use only. Not for use in diagnostic procedures.
Need a customised solution?
Optional accessories are available for purchase separately. If your order needs a customised configuration, our technical specialists will follow up with full details before your order is finalised. Contact us via the enquiry form or sales@lasec.com.
Installation, training and special delivery
Some products require installation, training, or special delivery (such as tail-lift trucks, extra labour, or hazardous material packaging). If your order needs this, we'll contact you with the additional cost before dispatch, so your final invoice may differ from the listed price.
Ordering from outside South Africa
Online ordering is currently limited to South African customers, but our full range is still available. Log an enquiry or email international@lasec.com and our international sales team will assist you through our traditional sales channels.
DISCLAIMER: All product images are for illustrative purposes only. Actual products may vary slightly in colours, controls, and visible features.
Availability:On Request
SKU
IREVCMT-K2
BrandRevvity
Product Overview
The Cellometer K2 is a dual-fluorescence and brightfield image cytometer built for laboratories working with complex and messy primary cell samples, including whole blood, peripheral blood, cord blood, and bone marrow. Powered by Matrix software, it calculates cell count, concentration, diameter, and percent viability in under 60 seconds from 10 µl of sample. The AO/PI dual-fluorescence method selectively counts nucleated cells, excluding red blood cells, platelets, and debris without a lysis step. Over 400 preloaded cell types and predefined assays reduce setup time and support consistent, reproducible results.
Key Performance Figures
- Results in: <60 seconds
- Min sample volume: 10 µl
- Cell size range: 4–90 µm
- Concentration range: 10⁵ – 10⁷cells/ml
- Cell types preloaded: 400+
- Images per sample: 1, 4 or 8
- Weight: 10.4 kg
The AO/PI Dual-Fluorescence Method
Dual-colour fluorescence is recommended over brightfield or Trypan blue methods for reliable viability analysis in primary cells. The K2 includes standard assays for AO/PI staining across a wide range of cell types.
Acridine Orange (AO): A nucleic acid-binding dye permeable to both live and dead cells. Stains all nucleated cells and generates green fluorescence. Live nucleated cells fluoresce green.
Propidium Iodide (PI): Enters only dead cells with compromised membranes. Stains dead nucleated cells red. Where both AO and PI are present, PI quenches AO; dead nucleated cells fluoresce red.
RBC / debris exclusion: Mature mammalian red blood cells lack nuclei and produce no fluorescent signal. Platelets and debris are similarly excluded. No lysis step is required, saving time and reducing sample preparation complexity.
Instrument Capabilities
Complex Sample Handling: Designed for hepatocytes, stem cells, splenocytes, tumour suspensions, whole blood, peripheral blood, cord blood, and bone marrow. Pattern-recognition software declusters, identifies, and counts individual cells accurately in challenging preparations.
Multiple Fields of View: Capture 1, 4, or 8 images per sample. The default is 4 images, equivalent to 6 hemocytometer quadrants. 8 images are equivalent to 12 quadrants, improving counting dynamic range and result reliability at low or high concentrations.
Predefined And Customisable Reports: Export results as CSV, Excel, Word, or PDF. Default reports are available for common assays; custom reports can include graphs, images, charts, and tables. Statistical analysis parameters include average, variance, min/max, and standard deviation of cell size.
Disposable Counting Slides: Two disposable slide formats are available, one with protective coverings on both sides and a ready-to-use format in microscope slide boxes. No washing is required between uses, reducing contamination risk and biohazard exposure.
Predefined Assays
- Viability
- Apoptosis
- Transfection efficiency
Compatible Sample Types
- Hepatocytes
- Stem cells
- Splenocytes
- Tumour suspensions
- Whole blood
- Peripheral blood
- Cord blood
- Bone marrow
- Primary cells
- Cell lines
- Insect cells
- Fragile cells
Fluorescence Optics Modules
Excitation 470 nm / Emission 535 nm
- Acridine Orange (AO)
- CFDA
- FITC
Excitation 540 nm / Emission 660 nm
- Propidium Iodide (PI)
- Ethidium Bromide
- 7-AAD
Both modules are included as standard.
21 CFR Part 11 Compliance Module
- User login with passwords and user-assigned permissions
- Audit trail and error log files
- Electronic signatures
What Is Included
- Cellometer K2 instrument
- Cellometer software
- Two standard fluorescence optics modules
- Power supply
- USB 2.0 connection cable
- Phone and online setup support
PC Minimum Requirements
Applicable when purchasing the Cellometer K2 without the optional PC laptop accessory.
| Operating system | Windows 11, 64-bit |
| Processor | Intel Core i7, 2.0 GHz |
| RAM | 16 GB or higher |
| GPU | NVIDIA P1000 or equivalent / better |
| Dimensions (W x D x H) | 15.2 cm x 21.6 cm x 35.6 cm (6.0 in x 8.5 in x 14.0 in) |
| Weight | 10.4 kg (23.0 lbs) |
| Cell size range | 4–90 µm |
| Concentration range | 10ⁿ – 10⁷ cells/ml |
| Power input | 100–240 V AC, 50/60 Hz, 1.0 A |
| Power output to instrument | 12 V DC, 3.34 A |
| Connectivity | USB 2.0 |
| Imaging modality | Fluorescence, brightfield |
| Technology | Matrix software |
| 21 CFR Part 11 ready | Yes (optional module) |
| Unit size | 1 each |
For research use only. Not for use in diagnostic procedures. A PC laptop is available as an optional accessory.
| Brand | Revvity |
|---|
Write Your Own Review
Related Products By Brand
Enquire
Enquire
Enquire
Enquire
Enquire