Cellometer Ascend Automated Cell Counter
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- Imaging modality: Dual fluorescence and brightfield
- Max samples per run: 8 (3-chamber or 8-chamber slide options)
- Results in: Under 60 seconds
- Minimum sample volume: 10 µl per chamber (5 µl cell sample when stained 1:1 with AOPI)
- Preloaded cell types: Over 400
- Predefined assays: Viability, apoptosis, transfection efficiency, nuclei counting
- Compatible samples: Cultured cell lines, PBMCs, primary cells, whole blood, bone marrow, isolated nuclei
- Dimensions (W x D x H): 25.1cm x 33.0 cm x 47.5 cm
- Weight: 10.88kg
- Technology: Matrix software with glove-friendly tilting touchscreen
- 21 CFR Part 11: Optional module available
For research use only. Not for use in diagnostic procedures.
Need a customised solution?
Optional accessories are available for purchase separately. If your order needs a customised configuration, our technical specialists will follow up with full details before your order is finalised. Contact us via the enquiry form or sales@lasec.com.
Installation, training and special delivery
Some products require installation, training, or special delivery (such as tail-lift trucks, extra labour, or hazardous material packaging). If your order needs this, we'll contact you with the additional cost before dispatch, so your final invoice may differ from the listed price.
Ordering from outside South Africa
Online ordering is currently limited to South African customers, but our full range is still available. Log an enquiry or email international@lasec.com and our international sales team will assist you through our traditional sales channels.
DISCLAIMER: All product images are for illustrative purposes only. Actual products may vary slightly in colours, controls, and visible features.
Availability:On Request
SKU
IREVCMT-ASD
BrandRevvity
Product Overview
The Cellometer Ascend is a benchtop automated cell counter combining dual-fluorescence and brightfield imaging for fast, reliable cell counts from small sample volumes. Powered by Revvity's Matrix software, it offers autofocus, automatic slide detection, and pre-loaded protocols for over 400 cell types. Designed to reduce operator-dependent variability, it delivers size, count, concentration, and viability results in under 60 seconds from as little as 10 µl of sample.
Key Performance Figures
- Max samples per run: 8
- Results in: <60 seconds
- Min sample volume: 10 µl
- Cell types preloaded: 400+
- Images per sample: 2, 4 or 8
- Weight: 10.9 kg
Instrument Capabilities
Dual Fluorescence Imaging: Brightfield and dual fluorescent channels enable accurate viability measurement in messy samples, including whole blood, PBMCs, and debris-heavy preparations. Nucleated cells are counted while red blood cells and debris are excluded without the need for lysis.
Automatic Focus: Two proprietary autofocus methods ensure consistent image quality across samples without requiring manual adjustment before each count.
Slide Detection: Automatic slide detection identifies which chambers contain samples. Load as few or as many samples as needed. Unused chambers can be saved for later use within the same slide.
Matrix Software: Glove-friendly tilting touchscreen with an intuitive interface. Built-in predefined assays, favourites feature for commonly used settings, and over 400 preloaded cell types with optimised parameters.
Multiple Fields of View: Capture 2, 4, or 8 images per sample to increase counting accuracy across a representative area of the well.
Nuclei Counting: Pre-set protocol for counting isolated nuclei, developed for samples prepared for single-cell sequencing workflows.
Predefined Assays
- Viability
- Apoptosis
- Transfection efficiency
- Nuclei counting
Compatible Sample Types
- Cultured cell lines
- PBMCs
- Primary cells
- Whole blood
- Bone marrow
- Isolated nuclei
Slide Options
- 3-chamber slide (ASD-CHM3) — loading volume 20 µl of stained sample per chamber
- 8-chamber slide (ASD-CHM8) — loading volume 10 µl of stained sample per chamber
- Typical staining ratio is 1:1 with AOPI dual-fluorescent viability dye, enabling a count from as little as 5 µl of cell sample
21 CFR Part 11 Compliance Module
- User login with passwords and user-assigned permissions
- Audit trail and error log files
- Electronic signatures
| Dimensions (W x D x H) | 25.1 cm x 33.0 cm x 47.5 cm (9.9 in x 13.0 in x 18.7 in) |
| Weight | 10.88 kg |
| Imaging modality | Fluorescence, brightfield |
| Technology | Matrix software |
| 21 CFR Part 11 ready | Yes (optional module) |
| Automation compatible | Yes |
| Unit size | 1 each |
For research use only. Not for use in diagnostic procedures.
Frequently Asked Questions (FAQs): Cellometer™ Ascend™ Automated Cell Counter
Q: How much volume of my sample do I need to perform a count?
A: The loading volume of the stained sample in ASD-CHM3 slides is 20 µl. ASD-CHM8 slides require 10 µl of sample. Typically, you will stain your cell sample 1:1 with AOPI dual-fluorescent viability dye, so you can perform a cell count with as little as 5 µl of cell sample.
Q: Can I save the slide for later if I only have one sample to count?
A: Yes, load the counting chamber with as many or as few samples as you want to count. The Cellometer Ascend software will allow you to indicate which chambers contain samples you wish to count, and the rest of the slide can be saved for later use.
Q: How does the Ascend count challenging or messy cell types?
A: The Cellometer Ascend is designed to identify live and dead nucleated cells, even in very messy samples with RBCs and debris or in irregular-shaped cell populations. By using the AOPI dual-fluorescence viability dye, only nucleated cells are counted, and RBCs and debris are ignored (this method is so specific, it even works in whole blood without lysing!). The Cellometer Ascend will show you the images it captured and which cells were counted so that you can visually verify the results and trust your data.
Q: Can the Ascend count nuclei for downstream single-cell sequencing?
A: Yes, the Cellometer Ascend is designed to count isolated nuclei and comes with a pre-set protocol for nuclei counting, which was created based on samples prepared for single-cell sequencing.
Q: Do I have to manually adjust the focus on the Ascend?
A: Yes, the Cellometer Ascend is designed to count isolated nuclei and comes with a pre-set protocol for nuclei counting, which was created based on samples prepared for single-cell sequencing.
| Brand | Revvity |
|---|
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