Cellaca PLX High-Throughput Image Cytometer
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- Imaging modality: Brightfield and 5 fluorescent channels (Blue, Green, Red, Far Red); 13 total fluorescent channels, 6 per scan
- Batch throughput: Up to 24 samples in under 5 minutes
- Single-sample 4-channel scan: 1 minute
- Minimum sample volume: 15 µl (low-fluorescence slides) / 50 µl (counting plates)
- Cell size range: 5–80 µm
- Concentration range: 1×10⁵ – 1×10⁷ cells/ml
- Excitation LEDs: 370, 475, 531, 628 nm
- Emission filters: 452, 534, 605, 655, 692 nm
- Key compatible dyes: Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, PE, APC, Annexin V, 7-AAD, KIRAVIA Blue 520
- Available assay kits: Immune cell phenotyping (CD3/CD4/CD8), apoptosis detection, high-throughput viability, fluorescent protein analysis
- Platform: Non-fluidic, no daily maintenance, no decontamination between runs
- Automation compatible: Yes (API; optional automation licence)
- 21 CFR Part 11: Optional module available
- IQ/OQ option: Yes
- Multi-language support: Over 7,000 language settings
- Dimensions (W x D x H): 33 cm x 33 cm x 41 cm
- Weight: 20 kg
For research use only. Not for use in diagnostic procedures.
Need a customised solution?
Optional accessories are available for purchase separately. If your order needs a customised configuration, our technical specialists will follow up with full details before your order is finalised. Contact us via the enquiry form or sales@lasec.com.
Installation, training and special delivery
Some products require installation, training, or special delivery (such as tail-lift trucks, extra labour, or hazardous material packaging). If your order needs this, we'll contact you with the additional cost before dispatch, so your final invoice may differ from the listed price.
Ordering from outside South Africa
Online ordering is currently limited to South African customers, but our full range is still available. Log an enquiry or email international@lasec.com and our international sales team will assist you through our traditional sales channels.
DISCLAIMER: All product images are for illustrative purposes only. Actual products may vary slightly in colours, controls, and visible features.
Availability:On Request
SKU
IREVPLX-SYS1
BrandRevvity
Product Overview
The Cellaca PLX is a high-throughput image cytometer with brightfield and five fluorescent channels, designed for laboratories that need to move beyond simple cell counting into multiplexed cell analysis. It processes up to 24 samples in under 5 minutes using dual fluorescence and brightfield imaging and completes a single-sample four-channel multiplex scan in 1 minute. From routine viability measurements to immunophenotyping with CD3, CD4, and CD8 surface markers, apoptosis detection, and fluorescent protein analysis, the PLX delivers flow-like data at the lab bench from as little as 15 µl of sample. The non-fluidic design requires no daily start-up or shut-down cycles, no washing, and no decontamination of the instrument between runs.
Key Performance Figures
- Batch throughput: 24 samples <5 min
- 4-channel scan: 1 min per sample
- Min sample volume: 15 µl (slides)
- Fluorescent channels: 13 (6 per scan)
- Cell size range: 5–80 µm
- Weight: 20 kg
Instrument Capabilities
High-Throughput Batch Processing: Run up to 24 samples using dual fluorescence and brightfield imaging in under 5 minutes. The slide holder accommodates 4 slides (8 samples) per experimental run. Counting plates accept multi-channel pipettes for fast sample preparation.
Multiplexed Cell Analysis: Four-channel multiplex scans are complete in 1 minute per sample. Dedicated reagent kits combine surface marker detection, viability, and apoptosis readouts in a single assay, reducing time to downstream processing.
Immunophenotyping at the Bench: Immunophenotyping kits combine CD3, CD4, and CD8 surface marker detection with cell viability measurement in a single multiplex assay. Cell purity checks are performed without the need for a flow cytometer.
Non-Fluidic, Maintenance-Free Design: No internal fluidics means no clogging, no liquid waste, no daily start-up or shut-down cycles, and no instrument decontamination between runs. Low-fluorescence consumable slides provide a self-contained sample environment.
Complex Sample Handling: The AO/PI dual-fluorescence method counts nucleated cells selectively, excluding red blood cells, platelets, and debris without a lysis step. Works in whole blood. Counts both isolated nuclei and intact cells.
Automation and Integration: Compatible with robotic integration and automated liquid handling systems via API. An optional automation licence add-on is available. Multi-language support across over 7,000 language settings facilitates global data transfer.
Available Assay Kits
- Immune cell phenotyping
- Apoptosis detection
- High-throughput cell viability
- Fluorescent protein analysis
21 CFR Part 11 Compliance Module
- Audit trails, time stamping, user access control, and electronic signatures
- File locking and change tracking
- Customisable user roles with multiple configurations
- Closed-loop database system for data integrity and security
Cellaca PLX vs Cellaca MX at a Glance
The Cellaca instrument line covers two tiers of capability. Both share the same physical footprint and are automation-compatible
| Cellaca PLX | Cellaca MX (MX-AOPI) | |
|---|---|---|
| Channels | Brightfield, Blue, Green, Red, Far Red | Brightfield, Green, Red |
| Number of fluorescent channels | 13 (6 per scan) | 3 (2 per scan) |
| Excitation LED | 370 nm, 475 nm, 531 nm and 628 nm | 470 nm, 527nm |
| Emissions Filters | 452 nm, 534 nm, 605 nm, 655 nm and 692 nm | 534 nm, 655 nm |
| Commonly Used Compatible Dyes | Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, CMFDA, Calcein AM, 7AAD, Annexin V, PE, APC, KIRAVIA Blue 520™* | Trypan Blue, AO/PI, Calcein AM, Annexin V, Caspase 3/7 |
| Counting Speed | Fluorescence 4-channel scan in 1 minute (per plate) | Trypan Blue - 2 seconds Fluorescence 2-channel scan- Less than 8 seconds (per well) |
| Volume (per well) | 15 µL in slides 50 µL - 200 µL in counting plates | 50 µL - 200 µL in counting plates |
| Size/Diameter Range | 5 µm - 80 µm | 5 µm - 80 µm |
| Concentration Range | 1x105 - 1x107 cell/mL | 1x105 - 1x107 cell/mL |
| IQ/OQ Option | Yes | Yes |
| 21 CFR Part 11 Ready Option | Yes | Yes |
| Automation Compatible | Yes | Yes |
| Dimensions | 13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm) | 13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm) |
| Weight | 42 lbs (19 kg) | 42 lbs (19 kg) |
| Dimensions (W x D x H) | 33 cm x 33 cm x 41 cm (13 in x 13 in x 16 in) |
| Weight | 20 kg (42 lbs) |
| Cell size range | 5–80 µm |
| Concentration range | 1×105 – 1×107 cells/ml |
| Sample volume — slides | 15 µl minimum |
| Sample volume — counting plates | 50–200 µl |
| Imaging modality | Fluorescence (5 channels), brightfield |
| Technology | Matrix software |
| 21 CFR Part 11 ready | Yes (optional module) |
| Automation compatible | Yes (API; optional automation licence) |
| IQ/OQ option | Yes |
| Multi-language support | Over 7,000 language settings |
| Unit size | 1 unit |
For research use only. Not for use in diagnostic procedures.
Frequently Asked Questions (FAQs): Cellaca PLX High-throughput Image Cytometer
Q: How much volume of my sample do I need to perform a count?
A: The minimum loading volume of the stained sample in the counting plate is 50 µl. Typically, you will stain your cell sample 1:1 with AOPI dual-fluorescent viability dye, so you can perform a cell count with as little as 25 µl of cell sample. For cell-based assays such as immunophenotyping or GFP/RFP, instead of the counting plate, you will use low-fluorescent background slides that only require 15 µl of sample. If you have a low-volume sample, you can perform a cell count in the slides as well.
Q: Can I save the plate for later if I only have a few samples to count?
A: Yes, load the counting plate with as many or as few samples as you want to count. The Cellaca software will allow you to indicate which wells contain samples you wish to count, and the rest of the plate can be saved for later use.
Q: Are the counting plates compatible with multi-channel pipettes?
A: Yes, the counting plates are compatible with multi-channel pipettes, making it fast and easy to stain and prep your samples. The Cellaca is also compatible with liquid handlers and automation platforms and has an optional automation license add-on, making it easy to scale up your throughput.
Q: What daily start-up or shut-down cycles do I need to run?
A: There are no daily start-up or shut-down cycles for the Cellaca because there are no internal fluidics and nothing that needs to get cleaned or could clog. The Cellaca uses LEDs as a light source. The only prep you need to do is turn on the instrument and start up the software, and the Cellaca is ready to count your samples right away.
Q: How does the Cellaca count challenging or messy cell types?
A: The Cellaca is designed to identify live and dead nucleated cells, even in very messy samples with RBCs and debris or in irregular-shaped cell populations. By using the AOPI dual-fluorescence viability dye, only nucleated cells are counted, and RBCs and debris are ignored (this method is so specific, it even works in whole blood without lysing!). The Cellaca will show you the images it captured and which cells were counted so that you can visually verify the results and trust your data.
| Brand | Revvity |
|---|
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