Celigo Image Cytometer
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- Imaging modality: Brightfield (LED-enhanced) and 4 fluorescent channels (Blue, Green, Red, Far-Red)
- Key differentiator: Whole-well imaging with proprietary F-theta lens and galvanometric scanning mirrors, uniform edge-to-edge illumination without trypsinisation
- 384-well plate scan time: Under 2 minutes
- Per-channel scan time: Approximately 5 minutes per plate
- Resolution options: 1, 2, 4, or 8 µm/pixel
- Camera: Large chip CCD, 2024 x 2024 pixels
- Compatible plate formats: 6-well to 1536-well; T-25 and T-75 flasks; 10 cm dishes; glass slides (on request)
- Fluorescent channels: Blue (377/470 nm), Green (483/536 nm), Red (531/629 nm), Far-Red (628/688 nm)
- Key applications: Label-free adherent cell counting, 3D tumour model analysis, immuno-oncology (ADCC, NK cell, CAR-T), monoclonality verification, drug screening, virology, iPSC reprogramming, migration and invasion
- Automation compatible: Yes (optional licence; supports robotic systems, plate stackers, liquid handlers; >100 plates/day)
- 21 CFR Part 11: Optional module available
- Operating system: Windows 11
- Power supply: 110–220 V AC, 50/60 Hz
- Dimensions (W x D x H): 50.8 cm x 43.2 cm x 63.5 cm
- Weight: 53.0 kg
- Regulatory compliance: CE marking
For research use only. Not for use in diagnostic procedures.
Need a customised solution?
Optional accessories are available for purchase separately. If your order needs a customised configuration, our technical specialists will follow up with full details before your order is finalised. Contact us via the enquiry form or sales@lasec.com.
Installation, training and special delivery
Some products require installation, training, or special delivery (such as tail-lift trucks, extra labour, or hazardous material packaging). If your order needs this, we'll contact you with the additional cost before dispatch, so your final invoice may differ from the listed price.
Ordering from outside South Africa
Online ordering is currently limited to South African customers, but our full range is still available. Log an enquiry or email international@lasec.com and our international sales team will assist you through our traditional sales channels.
DISCLAIMER: All product images are for illustrative purposes only. Actual products may vary slightly in colours, controls, and visible features.
Availability:On Request
SKU
IREV200-BFFL-5C
BrandRevvity
Product Overview
The Celigo is a plate-based benchtop image cytometer that captures whole-well images across microplate formats from 6-well to 1536-well, including T-flasks and petri dishes, without removing cells from their culture environment. A proprietary F-theta scan lens and galvanometric scanning mirrors provide uniform illumination and flat, edge-to-edge image contrast across the full well area. Brightfield and four fluorescent channels support a broad range of applications from label-free adherent cell counting to complex immuno-oncology assays, 3D tumour model analysis, and monoclonality verification. A complete 384-well plate is imaged and analysed in under 2 minutes, with quantitative results available for each well as soon as it is captured.
Key Performance Figures
- 384-well plate scan: <2 minutes
- Per channel (whole well): ~5 min per plate
- Fluorescent channels: 4 + brightfield
- Resolution: 1–8 µm/pixel
- Camera: 2024×2024px CCD
- Weight: 53kg
Instrument Capabilities
Whole-Well Imaging with Uniform Illumination: The proprietary F-theta lens combined with galvanometric scanning mirrors delivers uniform illumination and superior edge-to-edge contrast across the full well area, including at well edges where conventional optics introduce distortion.
Label-Free Adherent Cell Analysis: Brightfield imaging quantifies adherent cells directly in the plate without trypsinisation, avoiding cell loss and preserving experimental conditions. Cells can be measured at multiple time points in the same vessel.
2D And 3D Culture Compatibility: Supports both adherent and suspension cells in 2D cultures as well as spheroids, embryoid bodies, patient-derived organoids, and other 3D models. Readouts include growth, viability, invasion, and migration.
High-Speed Concurrent Analysis: Scanning and analysis run concurrently. Quantitative results for the first well are available before the rest of the plate is scanned. A 384-well plate completes in under 2 minutes.
Monoclonality Verification: The Celigo Monoclonality Report detects and screens single cell-derived colonies across multiple time points, supporting single cell per well verification for cell line development workflows.
Open Image Export and AI Compatibility: Images export as .jpg or .tif in open format, individual well images or stitched whole-well images, with rule-based export filters. Compatible with Revvity's Image Artist platform (sold separately) for AI-assisted analysis, remote access, and multi-system data management.
Application Areas
3D models
- Tumour spheroid viability
- Growth inhibition
- Invasion into Matrigel
- 3D migration
- Embryoid bodies and PDOs
- 3D confrontation assay
- Clonogenic survival
Brightfield imaging
- Adherent cell growth tracking
- Suspension cell counting
- Embryoid bodies
- Colonies
- Spheroids
- Wound healing
- Morphology
Immuno-oncology
- ADCC
- Direct NK cell killing
- CAR-T cytotoxicity
- CDC
- Direct cell counting and visualisation
Virology
- Viral titer
- Viral infection
- Antibody neutralisation
- Transduction efficiency
- Cytopathic effect
iPSC reprogramming
- iPSC colony counting
- Embryoid body formation
- Fibroblast doubling
- Immunostaining for differentiation
Cell line development
- Single colony identification
- Single cell per well verification
- Monoclonality screening
- Transfection and transduction efficiency
Fluorescent imaging
- Cell cycle
- Cell health
- Internalisation and phagocytosis
- Co-culture
- Surface proteins and antibodies
- Transfection and transduction
- Apoptosis
- Migration
Migration and invasion
- Chemotaxis
- Wound healing
- Transwell invasion
- 3D invasion
Fluorescent Channel Specifications
| Channel | Excitation (nm) | Dichroic (nm) | Emission (nm) | Typical dyes |
|---|---|---|---|---|
| Blue | 377/50 | 409 | 470/22 | Hoechst, DAPI |
| Green | 483/32 | 506 | 536/40 | FITC, Calcein, GFP, AlexaFluor® 488 |
| Red | 531/40 | 593 | 629/53 | R-PE, PI, Texas Red, AlexaFluor® 568 |
| Far-Red | 628/40 | 660 | 688/31 | DRAQ5®, AlexaFluor® 647 |
Compatible Plate Formats
- 6-well
- 12-well
- 24-well
- 48-well
- 96-well
- 384-well
- 1536-well
- T-25 flask
- T-75 flask
- 10 cm dish
- Glass slides*
Black, white, and clear wall plates supported. *Slide and cell array plate profiles available upon request. Compatible with 6-, 12-, 24-, 48-, 96-, 384-, and 1536-well formats.
| Dimensions (W x D x H) | 50.8 cm x 43.2 cm x 63.5 cm (20.0 in x 17.0 in x 25.0 in) |
| Weight | 53.0 kg |
| Camera | Large chip CCD, 2024 x 2024 pixels |
| Resolution | 1, 2, 4, or 8 µm/pixel |
| Imaging channels | 1 brightfield (LED-enhanced) + 4 fluorescent (LED-based) |
| Optics | Proprietary F-theta lens; galvanometric scanning mirrors |
| 384-well plate scan time | Under 2 minutes |
| Power supply | 110–220 V AC, 50/60 Hz |
| Operating system | Windows 11 |
| Technology | Celigo Pro software |
| 21 CFR Part 11 ready | Yes (optional module) |
| Automation compatible | Yes (optional automation licence; compatible with robotic systems, plate stackers, and liquid handlers) |
| Regulatory compliance | CE marking |
| Unit size | 1 unit |
For research use only. Not for use in diagnostic procedures. Image Artist platform is sold separately and is not included with the Celigo system. AlexaFluor, DRAQ5, SYTOX, and Cy are trademarks of their respective owners.
Frequently Asked Questions (FAQs): Celigo Image Cytometer
Q: What makes the Celigo distinct from conventional imaging systems?
A: The Celigo uses a large F-theta scan lens and galvanometric scanning mirrors to rapidly capture whole-well images with uniform, flat illumination from edge to edge. It is designed for rapid speed-to-data, providing fast high-quality imaging and built-in concurrent quantitative analysis making it suited to screening applications in 2D and 3D, rare population detection, viral infection studies, single cell documentation, and label-free or fluorescent cell-based assays directly in the plate without trypsinisation.
Q: How fast can the Celigo scan a plate?
A: A useful guideline is approximately 5 minutes per channel for whole-well images across an entire plate. A 384-well plate completes in under 2 minutes. Because scanning and analysis run concurrently, quantitative results for the first well are available as soon as that well is captured before the rest of the plate has finished scanning.
Q: Can Celigo images be analysed with AI or third-party software?
A: Yes. Images are exported in open .jpg or .tif format either as individual well images or stitched whole-well images and can be analysed in any software. Rule-based export filters allow selective export based on defined criteria. Revvity's Image Artist platform (sold separately) provides AI-assisted analysis, remote browser access, and centralised management of image data from Celigo and other imaging systems.
Q: What is the benefit of using the Celigo for drug screening?
A: The Celigo counts and analyses cells directly in the plate, enabling label-free proliferation tracking over the course of treatment followed by endpoint assays such as viability staining, cell cycle analysis, or apoptosis without trypsinising. In 3D models, readouts include size, migration, invasion, viability, and apoptosis. Rapid imaging and analysis support the use of multiple replicates for high-quality screening assays.
Q: Is the Celigo compatible with automation?
A: Yes. An optional automation licence enables integration with robotic systems, plate stackers, and liquid handlers, scaling throughput to over 100 plates per day. The Celigo has been integrated into Revvity automated workstations with robotic arms, automated incubators, and scheduling software. Third-party automation platforms are also supported.
| Brand | Revvity |
|---|
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