RAL 555 Kit

The RAL 555 Kit is a fast-acting variation of May-Grünwald Giemsa staining. It is a versatile staining kit that can serve in haematology (blood and bone marrow smears), parasitology, histology, spermiology, and cyto-bacteriology.

This staining technique enables, for example, the identification of malaria or the study of spermatozoa morphology (spermocytogram). Most commonly used in haematology, this kit offers a reliable and reproducible method for staining blood smears, achieving results in just 15 seconds.

How does the RAL 555 Kit work?

This kit consists of 3 ready-to-use bottles for a 3-step bath staining technique. The staining only requires insertion of the slide successively in the three different bottles for 5 seconds in each.

3 bottles for 3 functions:

• The first FIX-RAL 555 bottle allows you to fix cells on the slide.
• The second EOSINE-RAL 555 bottle contains an eosin solution, an acid stain with a special affinity for basic cellular elements such as the cytoplasm.
• The last BLUE-RAL 555 bottle contains a methylene blue dye, a basic stain fixing acid cellular elements like the nucleus and chromatin of cells.

The analysis of blood and bone marrow smears using the RAL 555 Kit is similar to that performed with Romanowsky classic staining.

Benefits:

• Gives a Reliable Result: One kit guarantees the staining of 230 slides with the same consistent quality for microscope analysis.
• Save Time: Easy 3-step protocol, allowing you to stain a blood smear in only 15 seconds and a bone marrow smear in 3 minutes. The ready-to-use reagents are time-saving for the technicians.
• Simplify Your Handling: To facilitate handling, the bottles have a wide neck, allowing you to directly insert the slides for the staining.
• Work Safely: The first step of fixation ensures the absence of cross-contamination. The ready-to-use reagents combined with short staining protocols limit exposure to vapours.

Applications:

• Haematology: The RAL 555 Kit, a fast variation of the May-Grünwald Giemsa technique, enables differential staining of medullary and blood smears.
• Cyto-Bacteriology: The RAL 555 Kit allows the cytological study of urine, liquids, and spinal fluid.
• Parasitology and Mycology: The RAL 555 Kit enables the detection of parasites and fungi on blood smears: tissular protozoa (Leishmania, Toxoplasma, Microsporidia), Cryptosporidium, Pneumocystis carinii, fungi contributing to deep mycosis, veterinary parasitology (Piroplasms, M. pachydermatis), Plasmodium, Trichomonas, and Microfilaria.
• Histo-Cytology: The RAL 555 Kit allows the cytological study of fixed and paraffin-embedded tissue sections, punctions (breast and deep organs), effusion fluids of serous membranes (pleura, peritoneum, etc.), and enables the detection of Helicobacter pylori on histological sections.