PureIT ExoZAP PCR CleanUp, 100 Reactions
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- Reactions: 100
- Pack size: 1 tube x 0.2 ml
- Purification type: Enzymatic PCR Cleanup
- Composition: Heat-labile Exonuclease I (HL-ExoI) and recombinant Shrimp Alkaline Phosphatase (rSAP)
- Cleanup protocol volume: 2 µl per 5 µl PCR product (scale proportionally for larger volumes)
- For Research Use Only. Not for use in diagnostic procedures.
- Other product sizes, combinations and customised solutions are available.
Need a customised solution?
Optional accessories are available for purchase separately. If your order needs a customised configuration, our technical specialists will follow up with full details before your order is finalised. Contact us via the enquiry form or sales@lasec.com.
Installation, training and special delivery
Some products require installation, training, or special delivery (such as tail-lift trucks, extra labour, or hazardous material packaging). If your order needs this, we'll contact you with the additional cost before dispatch, so your final invoice may differ from the listed price.
Ordering from outside South Africa
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Availability:On Request
SKU
BAMPA620601
BrandAmpliqon
Product Overview
PureIT ExoZAP PCR CleanUp is a two-enzyme formulation for the direct cleanup of amplified PCR products prior to downstream applications. It combines a heat-labile Exonuclease I (HL-ExoI) and a recombinant Shrimp Alkaline Phosphatase (rSAP) in a balanced mixture. Added directly to the amplified PCR product, it removes residual primers and single-stranded DNA, and inactivates excess dNTPs by dephosphorylation. No sample loss is observed after treatment because there is no need for transfer, spin columns, or magnetic beads.
Kit Features
- One-step cleanup is added directly to the mixture containing the amplified PCR product
- 5-minute total protocol with no need for spin columns or magnetic beads
- No sample loss observed after treatment
- Improves results in downstream applications, including DNA sequencing and SNP analysis
- Suitable for NGS library preparation protocols
Cleanup Process
Incubation of the amplified PCR products at 37 °C for a minimum of 2 minutes degrades primers and single-stranded DNA, while also deactivating excess dNTPs. Subsequent heating to 80 °C for 3 minutes fully inactivates the enzymes, leaving samples ready for downstream applications.
Downstream Applications
- DNA sequencing
- Primer extension experiments
- SNP analysis
- NGS library preparation
Protocol
Storage and Stability
Post-treatment storage: -20 °C
Long-term storage: -20 °C (expiry as stated on label)
Quality Control
PureIT ExoZAP PCR CleanUp is functionally tested by spiking a PCR product with dNTPs and primers, followed by Sanger sequencing via capillary electrophoresis. HL-ExoI and rSAP are each tested individually for double-stranded and single-stranded endonuclease activity.
| Brand | Ampliqon |
|---|---|
| Reaction Size | 100 Reactions |
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