NEXTFLEX® Next-Generation Sequencing (NGS) Library Preparation Kits
NEXTFLEX® Next-Generation Sequencing (NGS) Library Preparation Kits
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- Streamlined NGS library prep kits for Illumina® and Element Biosciences™ platforms.
- Supports DNA-seq, RNA-seq, small RNA-seq, targeted sequencing, and single-cell workflows.
- Compatible with automation for higher throughput and reduced hands-on time.
- Delivers high-quality, reproducible libraries for accurate, comprehensive sequencing data.
- Broad kit portfolio for whole-genome, amplicon, targeted resequencing, and 16S rRNA sequencing.
- Accessory products available (blockers, cleanup beads, ribodepletion reagents, barcodes) for workflow flexibility.
- Integrates seamlessly into existing lab protocols and automated liquid handling systems.
- Trusted technical support and verified protocols for reliable, efficient genomic workflows.
For research use only. Not for use in diagnostic procedures.
Availability:On Request
SKU
BREV-NEXTFLEXPREP
BrandRevvity
Streamlined, High-Quality NGS Library Preparation for Genomic Research
Overview
The NEXTFLEX® NGS Library Preparation Kits are engineered to deliver efficient, reliable, and high-quality library preparation for Illumina® and Element Biosciences™ sequencing platforms. These kits support a broad range of DNA and RNA sequencing applications, providing seamless integration with automated liquid handling systems to increase throughput, reduce hands-on time, and enhance reproducibility in modern genomics laboratories.
NEXTFLEX® kits simplify the critical NGS library preparation steps, including fragmentation, end repair, adapter ligation, and enrichment, enabling researchers to obtain accurate and comprehensive sequencing data while maintaining consistency and robustness across workflows.
Key Features and Benefits
Automation-Ready for Increased Throughput
Fully compatible with automated liquid handling systems, reducing manual pipetting, enhancing consistency, and accelerating library preparation workflows.
Platform Compatibility
Optimised for Illumina® and Element Biosciences™ sequencing systems for seamless integration into existing laboratory workflows.
High Performance and Reproducibility
Validated protocols deliver high-yield, high-integrity libraries, ensuring robust results for NGS, PCR, and other downstream analyses.
Accessory Compatibility
NEXTFLEX® accessory products—including Universal Blockers, NGS Cleanup Beads, ribodepletion reagents, and barcodes—are compatible with a wide range of library prep systems from Illumina™, NEB™, Watchmaker™, Twist™, and others, allowing for workflow enhancements without disrupting established protocols.
Cost-Effective Flexibility
Modular and versatile to adapt to evolving project needs while maintaining cost efficiency.
Comprehensive Application Coverage
NGS Adapter: Barcoded adapters for highly efficient multiplexing in NGS workflows.
Small RNA-Seq Library Preparation Kits: Gene silencing and post-transcriptional regulation of gene expression can be investigated by small RNA-seq, which allows for the profiling of all of the small RNA and miRNA present in samples.
RNA-Seq Library Preparation Kits: Designed for mRNA sequencing, total RNA sequencing, and strand-specific RNA sequencing, enabling detailed insights into gene expression and regulation
DNA-Seq Library Preparation Kits: Support whole-genome sequencing, targeted sequencing, and amplicon sequencing, facilitating the exploration of genetic variations and structural genomic changes
Targeted Sequencing: Targeted resequencing for a highly targeted approach that analyses specific genomic regions.
16S rRNA Sequencing: 16S amplicon panels are used to identify and compare bacteria present within an environmental sample.
Single-Cell DNA Sequencing: Supports amplification and library preparation from single-cell and low-input DNA templates, enabling precision genomics in limited samples.
Reproductive Genetic Research: Streamlined PGT-A preparation workflows.
Why Choose NEXTFLEX® NGS Library Preparation Kits?
- Accelerate Your Research with robust, automation-ready protocols.
- Ensure Consistency and Reproducibility across your library prep workflows.
- Expand Application Versatility from whole-genome sequencing to targeted and single-cell applications.
- Enhance Cost Efficiency without compromising quality.
- Leverage Full Technical Support from LASEC and Revvity’s experienced genomics specialists.
NEXTFLEX® Next-Generation Sequencing Library Preparation Kits enable high-quality, reproducible, and efficient library preparation, empowering researchers to achieve exceptional sequencing results across diverse genomic applications. Designed to support modern, high-throughput laboratories, NEXTFLEX® kits are your partner in advancing genomic discovery with confidence.
| Product | Description | Part Number | Learn More | Request a Quote |
|---|---|---|---|---|
| NEXTFLEX NGS Cleanup Beads | The NEXTFLEX® NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput. | NOVA-BEADS-1ML, NOVA-BEADS-5ML, NOVA-BEADS-50ML, NOVA-BEADS-500ML | Learn More | |
| NEXTFLEX™ HT Agrigenomics Low-pass WGS Kit | The NEXTFLEX™ HT Agrigenomics Low-pass WGS kit is designed for the highly efficient conversion of DNA for Low pass whole genome sequencing WGS or Skim-seq applications, from both high- and low-quality samples from breeders, into Illumina® and Element® compatible libraries and can be paired with analysis solutions from CURIO. Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research. | NOVA-5141-70, NOVA-5141-71, NOVA-5141-72, NOVA-5141-73, NOVA-5141-74 | Learn More | |
| NEXTFLEX Rapid XP v2 DNA-seq Kit for Metagenomics | The NEXTFLEX® Rapid XP V2 DNA-seq kit for Metagenomics offers a simplified DNA-seq library preparation workflow for whole genome metagenome sequencing (WGS) of microbial communities on Illumina® and Element Biosciences® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool. The kit is now bundled with the Cosmos-Hub analysis solution. | NOVA-5149-21S, NOVA-5149-22S, NOVA-5149-23S | Learn More | |
| NEXTFLEX UDI-UMI Barcodes | The NEXTFLEX™ UDI-UMI Barcodes consist of 96 unique dual-index (UDI) barcodes, each with a 10-base pair UDI and a 9-base pair unique molecular identifier (UMI). These UMI adapters are designed for multiplexing both DNA and RNA NGS libraries on all Illumina® and Element® sequencers and are compatible with any TruSeq® style library prep kit. | NOVA-734100, NOVA-734101, NOVA-734102, NOVA-734103 | Learn More | |
| NEXTFLEX Neo NGS RUO Panel 1 | NEXTFLEX Neo NGS RUO kits offer robust genome coverage to identify variants in over 390 genes. | 6100-01 | Learn More | |
| NEXTFLEX blood miRNA blockers | The NEXTFLEX® blood miRNA blockers are an oligonucleotide mix which reduce the abundance of specific microRNA abundant in total RNA isolated from human whole blood and plasma in small RNA libraries. They are intended to be used with the NEXTFLEX® Small RNA-Seq Kit v4. | NOVA-513103 | Learn More | |
| NEXTFLEX 16S V1-V3 rRNA Amplicon-seq Kits | Library preparation kits for 16S V1-V3 rRNA bacterial metagenomics analysis for Illumina® and Element Biosciences® NGS platforms. | NOVA-4202-02S, NOVA-4202-02, NOVA-4202-03S, NOVA-4202-03, NOVA-4202-04S, NOVA-4202-04, NOVA-4202-05S, NOVA-4202-05, NOVA-4202-06S, NOVA-4202-06, NOVA-4202-07S, NOVA-4202-07 | Learn More | |
| NEXTFLEX Rapid XP V2 DNA-Seq Kit | The NEXTFLEX™ Rapid XP V2 DNA-Seq Kit is a fragmentation-based whole genome sequencing (WGS) library preparation workflow, taking samples from DNA to sequencing-ready libraries in as little as 2.5 hours. Proprietary NEXTFLEX normalization beads equalize library concentrations during the library prep process, so no separate quantitation or manual pooling is required. The protocol supports inputs from 100 pg to 1 µg and offers a PCR-free option for ≥ 250 ng, preserving library complexity and minimizing GC bias. Resulting libraries show uniform coverage and low duplicate rates on Illumina® and Element Biosciences™ AVITI platforms. | NOVA-5149-21, NOVA-5149-22, NOVA-5149-23 | Learn More | |
| NEXTFLEX RiboNaut rRNA Depletion Kit (Human / Mouse / Rat) | The NEXTFLEX® RiboNaut™ rRNA depletion kit (human / mouse / rat) is an effective method to remove rRNA contamination from mammalian total RNA while enabling labs to interrogate additional RNA species in a sample, not only limited to intact mRNAs. | NOVA-512961, NOVA-512962, NOVA-512964, NOVA-512963, NOVA-512965 | Learn More | |
| NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0 | The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, for sequencing on Illumina® or Element Biosciences® NGS platforms in two hours. | NOVA-5150-01, NOVA-5150-02 | Learn More | |
| NEXTFLEX Unique Dual Index Barcodes | The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters that can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for sequencing on Illumina® and Element® platforms. They are compatible with any library prep workflows that involve the ligation of adapters to adenylated fragments (TruSeq® style). | NOVA-514150, NOVA-514151, NOVA-514152, NOVA-514153, NOVA-514150-EVAL16, NOVA-514150-EVAL48 | Learn More | |
| NEXTFLEX Universal Blockers | The NEXTFLEX® Universal Blockers are short DNA oligos that block non-specific hybridization between adapter sequences, enhancing specificity and reducing sequencing costs. They are compatible with all NEXTFLEX® barcodes and all other ligation and tagmentation based workflows for Illumina® and Element® sequencing platforms. | NOVA-5143231, NOVA-5143232, NOVA-5143233 | Learn More | |
| NEXTFLEX Poly(A) Beads 2.0 | The NEXTFLEX® Poly(A) Beads 2.0 kit offers a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep with improved mRNA yields, low rRNA contamination, and a simple protocol. | NOVA-512991, NOVA-512992, NOVA-512994, NOVA-512993, NOVA-512995 | Learn More | |
| NEXTFLEX Rapid Directional RNA-Seq Kit 2.0 | The NEXTFLEX™ Rapid Directional RNA-seq library prep kit 2.0 produces libraries for Illumina® and Element Biosciences® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination. | NOVA-5198-01, NOVA-5198-02, NOVA-5198-03, NOVA-5198-43, NOVA-5198-53 | Learn More | |
| NEXTFLEX 16S V3 – V4 Amplicon-Seq Library Prep Kit | The NEXTFLEX® 16S V3 – V4 Amplicon-Seq Library Prep Kit prepares multiplexed amplicon libraries targeting hypervariable domains V3–V4 of microbial 16S rRNA genes. Libraries are Illumina® and Element® compatible with minimal cleanups and robust recovery. | NOVA-4204-03S, NOVA-4204-03, NOVA-4204-04S, NOVA-4204-04 | Learn More | |
| NEXTFLEX Rapid DNA-Seq Kit 2.0 | The NEXTFLEX™ Rapid DNA-Seq Kit 2.0 supports whole genome sequencing from 1 ng–1 µg of fragmented DNA. It enables fast Illumina® and Element® compatible library construction with low GC bias and automation-ready workflow. | NOVA-5188-01, NOVA-5188-02, NOVA-5188-03 | Learn More | |
| NEXTFLEX UDI Barcodes (1–1,536) | Multiplex up to 1,536 samples on a single flow cell with NEXTFLEX™ Unique Dual Index Barcodes (10NT UDIs). Each kit ships with up to 1,536 pre-validated i5/i7 index pairs that eliminate index hopping and are compatible with Illumina® platforms such as the NovaSeq® and Element’s AVITI™ systems. Off-the-shelf plate sets (containing between 16 to 1,536 unique adapters) let you start small and scale to population-level projects without requiring custom ordering or re-balancing. | NOVA-634100-EVAL16, NOVA-634100-EVAL24, NOVA-634100-EVAL48, NOVA-634101, NOVA-634102, NOVA-634103, NOVA-634104, NOVA-634105, NOVA-634106, NOVA-634107, NOVA-634108, NOVA-634109, NOVA-634110, NOVA-634111, NOVA-634112, NOVA-634113, NOVA-634114, NOVA-634115, NOVA-634116, NOVA-634100, NOVA-534101, NOVA-534102, NOVA-534103, NOVA-534104, NOVA-534100 | Learn More | |
| NEXTFLEX Small RNA Sequencing Kit V4 | The NEXTFLEX™ Small RNA-seq Kit v4 is a gel free small RNA sequencing solution that builds high complexity miRNA libraries from as little as 1 ng of total RNA. The kit reduces adapter ligation bias and adapter dimers, in 6 hours for biomarker discovery, exosome miRNA profiling, low input Ribo-seq, and other applications. | NOVA-5132-31, NOVA-5132-31C, NOVA-5132-32, NOVA-5132-41, NOVA-5132-42, NOVA-5132-43, NOVA-5132-44 | Learn More | |
| NEXTFLEX RNA-seq 2.0 UDI barcodes | The NEXTFLEX® RNA-seq 2.0 UDI barcodes are 384 barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. | NOVA-512920-EVAL16, NOVA-512920-EVAL48, NOVA-512920, NOVA-512921, NOVA-512922, NOVA-512923 | Learn More |
Frequently Asked Questions (FAQs): NEXTFLEX® NGS Library Preparation Kits
Q: What are NEXTFLEX® NGS Library Preparation Kits used for?
A: They are used for preparing high-quality DNA and RNA libraries for next-generation sequencing on Illumina® and Element Biosciences™ platforms, supporting a range of workflows including DNA-seq, RNA-seq, small RNA-seq, targeted sequencing, and single-cell applications.
Q: What sequencing platforms are these kits compatible with?
A: NEXTFLEX® kits are optimized for Illumina® and Element Biosciences™ sequencing systems and integrate seamlessly into existing lab protocols.
Q: Are NEXTFLEX® kits compatible with automation?
A: Yes, they are fully compatible with automated liquid handling systems to reduce hands-on time and increase throughput while maintaining consistency.
Q: What applications can NEXTFLEX® kits support?
A: They support whole-genome sequencing, amplicon sequencing, targeted resequencing, 16S rRNA sequencing, single-cell sequencing, small RNA-seq, and RNA-seq for comprehensive genomic research.
Q: What workflows do these kits streamline?
A: NEXTFLEX® kits simplify fragmentation, end repair, adapter ligation, and enrichment steps in NGS library preparation, ensuring accurate, reproducible sequencing data.
Q: What are the benefits of using NEXTFLEX® NGS Library Preparation Kits?
A: Benefits include high performance and reproducibility, reduced manual pipetting, cost-effective flexibility, compatibility with a broad range of workflows, and enhanced throughput in modern genomics labs.
Q: Are accessory products available for these kits?
A: Yes, accessories such as Universal Blockers, NGS Cleanup Beads, ribodepletion reagents, and barcodes are available for workflow flexibility and are compatible with Illumina™, NEB™, Watchmaker™, Twist™, and other library prep systems.
Q: Are the NEXTFLEX® kits suitable for single-cell DNA sequencing?
A: Yes, they support library preparation and amplification from single-cell and low-input DNA templates, ideal for precision genomics on limited samples.
Q: Can these kits be used for reproductive genetic research?
A: Yes, they support streamlined PGT-A preparation workflows for reproductive genetic research.
Q: How do these kits enhance lab efficiency?
A: They reduce hands-on time, improve consistency, and support high-throughput workflows, making them ideal for labs seeking reliable, scalable genomic library preparation.
Q: Are these kits suitable for diagnostic procedures?
A: No, NEXTFLEX® kits are for research use only and are not intended for diagnostic procedures.
Q: What kind of technical support is available for these kits?
A: Full technical support is provided by LASEC and Revvity genomics specialists to assist with efficient implementation and troubleshooting in your genomic workflows.
| Brand | Revvity |
|---|
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