Genomic DNA Isolation Kit, 250 Preps

Norgen’s Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, bodily fluids, and nasal or throat swabs. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s resin binds DNA under high salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The purified genomic DNA is fully digestible with all restriction enzymes tested and is completely compatible with PCR and Southern Blot analysis.

The Genomic DNA Isolation Kit allows for the isolation of genomic DNA from various types of animal tissues or cell samples. In addition, a protocol is also provided for the purification of viral DNA. In all cases, the genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the sample being processed. The protocol can be completed in approximately 80 minutes (including incubation time).

Genomic DNA Isolation Kit Components, 250 Preps:

• Digestion Buffer A: 5 x 25 mL
  
• Buffer SK: 5 x 30 mL
  
• Wash Solution A: 5 x 18 mL
  
• Elution Buffer B: 5 x 30 mL
  
• Proteinase K: 5 x 12 mg
  
• Spin Columns: 250
  
• Collection Tubes: 250
  
• Elution tubes (1.7 mL): 250
  
• Product Insert: 1

Storage Conditions and Product Stability

The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature.