CFX Opus 96 Real-Time PCR System

CFX Opus 96 Real-Time PCR System

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96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables.

Availability:On Request

CFX Opus is the newest real-time PCR system in Bio-Rad's robust CFX family. The CFX Opus 96 Real-Time PCR System can analyze 96 wells and offers a number of new features, including:

  • More uniform thermal performance
  • Expanded connectivity - Wi-Fi, Ethernet, and USB
  • Cloud connectivity: Operate CFX Opus on our cloud platform or with desktop CFX Maestro Software
  • Network storage drive access for excellent data management
  • Shuttle optical system yields consistent optical measurements across your sample plate

Applications and Uses for the CFX Opus Real-Time PCR Systems

  • Gene expression without requiring ROX
  • End-point analysis for sequence detection
  • Protein thermal shift assays
  • Assay optimization with thermal gradient
Thermal Cycler
Maximum ramp rate, °C/sec5
Average ramp rate, °C/sec3.3
Heating and cooling methodPeltier
Lid, °C30–110
Range, °C4–100
Accuracy, °C±0.2 of programmed target at 90°C
Uniformity, °C±0.3 well-to-well after 10 sec of arrival at target
Operational range, °C30–100
Programmable span, °C1–24
Optical Detection
Excitation6 filtered LEDs
Detection6 filtered photodiodes
Range of excitation wavelengths, nm450–684
Range of emission wavelengths, nm510–730
SensitivityDetects 1 copy of target sequence in human genomic DNA
Dynamic range10 orders of magnitude
Scan Time
All channels, sec12
FAM/SYBR® Green only, sec
Operating systemWindows 10
Multiplex analysisUp to 5 targets per well
Sample capacity, wells96
Sample size, µl1–50 (10–50 recommended)
Communication interfaceUSB 2.0
Electrical approvalsIEC, CE
Dimensions (W x D x H), cm/in33x56x36 / 13x22x14
Weight, kg/lb22/48
More Information
Brand Bio-Rad
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