CFX Duet Real-Time PCR System
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A PCR detection system with 96-well block and 2-color detection plus FRET. Includes CFX Duet base unit and cables.
Note: Optional accessories are available and can be purchased separately.
Optional accessories are available for purchase separately. If you require a customised solution, please contact our technical specialists by selecting the "Add to enquiry" button and using our enquiry form or at sales@lasec.com.
Installation, training and special delivery requirements, such as the need for tail-lift trucks and additional labour and installation for large equipment and hazardous material (Hazmat) packaging, are chargeable in addition to standard freight and will be quoted separately before dispatch.
Online ordering is currently limited to South African customers, but our full range is still available. If you are ordering from outside South Africa, please log an enquiry or email internationallabsales@lasec.com, and our international sales team will gladly assist you through our traditional sales channels.
Availability:On Request
SKU
IBRD12016265
BrandBio-Rad
The CFX Duet Real-Time PCR System makes the power and consistency of Bio-Rad qPCR available to almost any lab. The CFX Duet System is a fresh, modern system for small and more economical labs with its improved thermal performance and proprietary, accurate optical shuttle system. The new compact, cost-effective system will enable you to make discoveries in the convenience of your laboratory without the need for sharing with your neighbors or other departments.
Features and Benefits
- Quick system set-up — easy installation and factory-calibrated optics
- Bio-Rad's best thermal uniformity and accuracy to date
- Uniform optical system free of regular calibration requirements
- Audit and e-signature compatibility with CFX Maestro Security Edition Software
- Eliminates the need for a reference/normalizing dye, the CFX Duet System can perform duplex assays including genotyping
Applications and Uses
- Gene expression without requiring ROX
- End-point analysis for sequence detection
- Protein thermal shift assays
- Assay optimization with thermal gradient
- Genotyping
| Thermal Cycler | |
|---|---|
| Maximum ramp rate, °C/sec | 5 |
| Average ramp rate, °C/sec | 3.3 |
| Heating and cooling method | Peltier |
| Lid, °C | 30–110 |
| Temperature | |
| Accuracy, °C | ±0.2 of programmed target at 90°C |
| Uniformity, °C | ±0.3 well-to-well after 10 sec of arrival at target |
| Gradient | |
| Operational range, °C | 30–100 |
| Programmable span, °C | 1–24 |
| Optical Detection | |
| Excitation | 3 filtered LEDs |
| Detection | 3 filtered photodiodes |
| Range of excitation/emission wavelengths, nm | 450–580 |
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA |
| Dynamic range | 10 orders of magnitude |
| Scan Time | |
| All channels, sec | 12 |
| FAM/SYBR® Green only, sec | 3 |
| Software | |
| Operating systems | Windows 10 and up |
| Multiplex analysis | Up to 2 targets per well |
| System | |
| Sample capacity, wells | 96 |
| Sample size, µl | 1–50 (10–50 recommended) |
| Communication interface | USB 2.0 |
| Electrical approvals | IEC, CE |
| Dimensions (W x D x H), cm/in | 33x56x36 / 13x22x14 |
| Weight, kg/lb | 22/48 |
| Brand | Bio-Rad |
|---|
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