Norgen’s Total RNA Purification Kits 96-Well (Deep Well Format) provides a rapid method for the high-throughput isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast, fungi, and plants. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components, such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications, including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Advantages of the Total RNA Purification Kits, 96-Well:
Total RNA Purification Kits, 96-Well Components, 6 x 96-well plates:
Kit Specifications | |
Maximum Binding Capacity Per Well | 50 μg |
Maximum Loading Volume Per Well | 500 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Maximum Amount of Starting Material | |
Animal Cells | 1 x 106 cells |
Animal Tissues | 10 mg |
Blood | 100 μL |
Plasma/Serum | 150 μL |
Bacteria | 1 x 109 cells |
Yeast | 1 x 108 cells |
Fungi | 40 mg |
Plant Tissues | 40 mg |
Time to Complete 96 Purifications | 30 minutes |
Average Yields* | |
HeLa Cells (1 x 106 cells) | 15 μg |
E. coli (1 x 109 cells) | 50 μg |
* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Brand | Norgen Biotek Corp. |
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