Total RNA Purification Kits, 50 Preps

Total RNA Purification Kits, 50 Preps

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  • Format: Spin Column
  • Size: 50 preps
  • Total RNA Purification Kits for the rapid purification of total RNA, including microRNA, without the use of phenol
  • Fast and easy processing using rapid spin-column format
  • Isolate total RNA, from large rRNA down to microRNA (miRNA)
  • No phenol or chloroform extractions
  • Isolate high-quality total RNA from a variety of sources
  • RNA can be isolated and detected from as little as a single animal cell
  • For research use only and NOT intended for in vitro diagnostics.
Availability:On Request
SKU
BNOR17200

These Total RNA Purification Kits are suitable for the isolation of total RNA from a range of samples, including cells, bacteria, yeast, viruses, and bodily fluids, including plasma/serum, blood, saliva, CSF, and more. Extract high-quality and pure RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more. These kits purify all sizes of RNA, from large mRNA and lncRNA down to microRNA (miRNA), in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate, irrespective of size. Moreover, when the RNA sequences are small (e.g., miRNA), the column binds small RNAs regardless of their GC content.

Advantages of Total RNA Purification Kits:

  • Extract high-quality and quantity total RNA, including miRNA
  • No phenol step required; isolate all RNA in one fraction
  • Bind and elute all RNA, irrespective of size or GC content, without bias
  • Very sensitive and linear down to a few cells without the need for carrier RNA
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications, including small-RNA sequencing.
  • Available in a variety of formats to suit your needs
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

  • Elution Volume: 50 - 100 µL
  • Plasma/Serum: 4 - 10 mL
  • Urine: 11 - 30 mL
  • Cell-Culture Media: 20 - 35 mL

Total RNA Purification Kit Components, 50 Preps:

  • Buffer RL:  40 mL
  • Wash Solution A: 38 mL
  • Elution Solution A: 6 mL
  • Mini Spin Columns: 50
  • Collection Tubes: 50
  • Elution tubes (1.7 mL): 50
  • Product Insert: 1
    BNOR17200 - Total RNA Purification Kits, 50 Preps Specifications
    Kit Specifications
    Maximum Binding CapacityUp to 50 μg RNA
    Maximum Loading Volume650 μL
    Size of RNA PurifiedAll sizes, including small RNA (< 200 nt)
    Maximum Amount of Starting Material
    Animal Cells3 x 106 cells
    Animal Tissues10 mg (for most tissues*)
    Blood100 μL
    Plasma/Serum200 μL
    Bacteria1 x 109 cells
    Yeast1 x 108 cells
    Fungi50 mg
    Plant Tissues50 mg
    Time to Complete 10 Purifications20 minutes
    Average Yield
    HeLa Cells (1 x 106 cells)15 μg
    E. coli (1 x 109 cells)50 μg
    More Information
    Brand Norgen Biotek Corp.
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