These Total RNA Purification Kits are suitable for the isolation of total RNA from a range of samples, including cells, bacteria, yeast, viruses, and bodily fluids, including plasma/serum, blood, saliva, CSF, and more. Extract high-quality and pure RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more. These kits purify all sizes of RNA, from large mRNA and lncRNA down to microRNA (miRNA), in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate, irrespective of size. Moreover, when the RNA sequences are small (e.g., miRNA), the column binds small RNAs regardless of their GC content.
Advantages of Total RNA Purification Kits:
Total RNA Purification Kit Components, 250 Preps:
Kit Specifications | |
Maximum Binding Capacity | Up to 50 μg RNA |
Maximum Loading Volume | 650 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Maximum Amount of Starting Material | |
Animal Cells | 3 x 106 cells |
Animal Tissues | 10 mg (for most tissues*) |
Blood | 100 μL |
Plasma/Serum | 200 μL |
Bacteria | 1 x 109 cells |
Yeast | 1 x 108 cells |
Fungi | 50 mg |
Plant Tissues | 50 mg |
Time to Complete 10 Purifications | 20 minutes |
Average Yield | |
HeLa Cells (1 x 106 cells) | 15 μg |
E. coli (1 x 109 cells) | 50 μg |
Brand | Norgen Biotek Corp. |
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