Norgen’s Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, bodily fluids, and nasal or throat swabs. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s resin binds DNA under high salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The purified genomic DNA is fully digestible with all restriction enzymes tested and is completely compatible with PCR and Southern Blot analysis.

The Genomic DNA Isolation Kit allows for the isolation of genomic DNA from various types of animal tissues or cell samples. In addition, a protocol is also provided for the purification of viral DNA. In all cases, the genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the sample being processed. The protocol can be completed in approximately 80 minutes (including incubation time).

Genomic DNA Isolation Kit Components, 100 Preps:

Digestion Buffer A: 2 x 25 mL
Buffer SK: 2 x 30 mL
Wash Solution A: 2 x 18 mL
Elution Buffer B: 2 x 30 mL
Proteinase K: 2 x 12 mg
Spin Columns: 100
Collection Tubes: 100
Elution tubes (1.7 mL): 100
Product Insert: 1

Storage Conditions and Product Stability

The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature.

BNOR24700 - Genomic DNA Isolation Kit, 50 Preps Specifications

Column Binding Capacity	25 µg
Average Yield:* HeLa Cells (1 x 10⁶) Tissue (from 10 mg kidney)	8 µg 10 µg
Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension	20 mg 3 x 10⁶ cells 150 µL 150 µL
Time to Complete 10 Purifications	80 minutes