96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables.
CFX Opus is the newest real-time PCR system in Bio-Rad's robust CFX family. The CFX Opus 96 Real-Time PCR System can analyze 96 wells and offers a number of new features, including:
Applications and Uses for the CFX Opus Real-Time PCR Systems
| Thermal Cycler | |
|---|---|
| Maximum ramp rate, °C/sec | 5 |
| Average ramp rate, °C/sec | 3.3 |
| Heating and cooling method | Peltier |
| Lid, °C | 30–110 |
| Temperature | |
| Range, °C | 4–100 |
| Accuracy, °C | ±0.2 of programmed target at 90°C |
| Uniformity, °C | ±0.3 well-to-well after 10 sec of arrival at target |
| Gradient | |
| Operational range, °C | 30–100 |
| Programmable span, °C | 1–24 |
| Optical Detection | |
| Excitation | 6 filtered LEDs |
| Detection | 6 filtered photodiodes |
| Range of excitation wavelengths, nm | 450–684 |
| Range of emission wavelengths, nm | 510–730 |
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA |
| Dynamic range | 10 orders of magnitude |
| Scan Time | |
| All channels, sec | 12 |
| FAM/SYBR® Green only, sec | |
| Software | |
| Operating system | Windows 10 |
| Multiplex analysis | Up to 5 targets per well |
| System | |
| Sample capacity, wells | 96 |
| Sample size, µl | 1–50 (10–50 recommended) |
| Communication interface | USB 2.0 |
| Electrical approvals | IEC, CE |
| Dimensions (W x D x H), cm/in | 33x56x36 / 13x22x14 |
| Weight, kg/lb | 22/48 |
| Brand | Bio-Rad |
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