Vibrio Cholerae TaqMan PCR Detection Kit Dx, 24 reactions

Norgen’s Vibrio cholerae TaqMan PCR Kit Dx is an in vitro diagnostic test for the detection and quantification of Vibrio cholera-specific DNA using real-time hybridization-fluorescence detection. The kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Vibrio cholerae TaqMan PCR Kit Dx comprises a master mix for target and PCR control detection, primer & probe mixes, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.

Vibrio cholerae TaqMan PCR Kit Dx Components, 24 reactions

• MDx TaqMan 2X PCR Master Mix Dx: 550 µL
• Vibrio cholerae Primer & Probe Mix Dx: 2 x 70 µL
• Vibrio cholerae Positive Control Dx – 200,000 copies/μL: 50 µL
• Nuclease-Free Water: 2 x 1.25 mL
• Product Insert: 1

Storage Conditions and Product Stability

The Vibrio cholerae TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. All kit components should be stored at -20°C upon arrival.