Norgen’s Stool DNA Isolation Kit Dx provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples. The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using Bead Tubes and a combination of chemical and physical homogenization and lysis, without the need of any phenol-chloroform extractions. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Stool DNA Isolation Kit Dx Components, 50 Preps

Lysis Solution: 60 mL
Lysis Additive: 6 mL
Binding Solution: 7 mL
Wash Solution I: 30 mL
Wash Solution II: 22 mL
Elution Buffer: 3 mL x 2
Bead Tube: 50
Mini Spin Columns: 50
Collection Tubes: 50
Elution tubes (1.7 mL): 50
Product Insert: 1

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature.

Maximum Stool Input	200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed	Frozen, fresh or preserved stool
Format	Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Elution Volume	50 μL
Time to Complete 10 Purifications	30 minutes
Applications	PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis.