Use the QX200 droplet reader to count PCR-positive and PCR-negative droplets with an optical detector and thereby complete the second step of the Droplet Digital PCR (ddPCR) workflow.
Droplets are sipped, and the singulator unpacks the emulsified droplets and streams them in a single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. For EvaGreen or probe-based digital PCR applications.
Required Instruments
QX200 Droplet Generator— partitions each ddPCR reaction mix into 20,000 nanoliter-sized droplets
PTC Tempo Deepwell Thermal Cycler (12015392) or C1000 Touch thermal cycler with 96-deep well reaction module (1851197)