The AutoDG Instrument simplifies the Droplet Digital PCR workflow, making digital PCR both scalable and practical. Prepare up to 96 samples at a time with minimal hands-on time and eliminate user-to-user variability.
The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical. The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time; it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.
Features and Benefits of the QX200 AutoDG Droplet Digital PCR System
Automated Droplet Generator: simplifies the ddPCR workflow and offers flexibility:
Generates droplets for 96 ddPCR reactions in under 45 minutes
Compatible with both the QX100 and QX200 Droplet Digital PCR Systems
A single AutoDG Instrument can supply 4–5 Droplet Readers continuously
Guides setup and loading with large, color touch-screen interface and LED lighting
User-definable plate layout with increments of 8 wells provides run-to-run flexibility
HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
Droplet Digital PCR Technology: the most precise and sensitive digital PCR solution for a wide variety of applications, providing:
Flexible digital PCR chemistry - optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
Nanoliter droplet partitioning - reduces bias from amplification efficiency differences and PCR inhibitors
Convenient assay design - standard curves are not required
Applications of the QX200 Droplet Digital PCR System
Cancer biomarker studies and copy number variation - measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. Predesigned, wet-lab validated PrimePCR ddPCR Assays are now available for mutation and copy number detection.
Pathogen detection - employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring
Next generation sequencing - perform absolute quantification of an NGS library without the use of a standard curve
Gene expression analysis - achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA
Environmental monitoring - test a wide variety of environmental samples such as soil and water
Food testing - perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods
Required Instruments
The QX200 AutoDG Droplet Digital PCR System requires the use of the C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.
Required Consumables
DG32 Cartridges for AutoDG
Automated Droplet Generation Oil for Probes
Automated Droplet Generation Oil for EvaGreen
Pipet Tips for AutoDG
Pipet Tip Waste Bin for AutoDG
Droplet Reader Oil
ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications
PrimePCR ddPCR Assays
ddPCR Supermixes for Probes (no dUTP)
QX200 ddPCR EvaGreen Supermix
ddPCR Supermix for Probes
One-Step RT-ddPCR Kit for Probes
Starting sample size, µl
20
Automated Droplet Generator capacity
1–96 samples/run
Droplets per 20 µl sample
20,000
QX200™ Droplet Reader capacity
1–96 samples
Sample illumination
Light-emitting diodes
Sample detection
Multi-pixel photon counter
Detection channels
FAM (EvaGreen), HEX (VIC)
Linear dynamic range
5 orders of magnitude
Precision
±10%
Droplets per 96-well plate, million
~1.5
Automated Droplet Generator dimensions (W x D x H)
Open: 66 x 56 x 89 cm (26 x 22 x 35 in) Closed: 66 x 56 x 66 cm (26 x 22 x 26 in)