Q-Extract DNA Extraction PCR Kit, 500 Reactions
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- Application: DNA Extraction & PCR (Genotyping)
- Reactions: 500
- Extraction Time: ~8 minutes
- Total Workflow: < 1.5 hours
- Sample Types: Mammalian tissue, plant leaves, saliva, bacteria
- PCR Ready: Yes (no purification required)
- Master Mix: 2× Taq DNA Polymerase RED (1.5 mM MgCl₂)
- Gel Loading: Direct (no loading dye required)
- Reaction Volume: 25 µL
- Storage: Extraction solution: -20 °C; Master mix: -20 °C
- For Research Use Only. Not for use in diagnostic procedures.
Optional accessories are available for purchase separately. If you require a customised solution, please contact our technical specialists by selecting the "Add to enquiry" button and using our enquiry form or at sales@lasec.com.
Installation, training and special delivery requirements, such as the need for tail-lift trucks and additional labour and installation for large equipment and hazardous material (Hazmat) packaging, are chargeable in addition to standard freight and will be quoted separately before dispatch.
Online ordering is currently limited to South African customers, but our full range is still available. If you are ordering from outside South Africa, please log an enquiry or email internationallabsales@lasec.com, and our international sales team will gladly assist you through our traditional sales channels.
Availability:On Request
SKU
BAMPA570004
BrandAmpliqon
Product Overview
The Q-Extract DNA Extraction PCR Kit is a rapid and efficient solution for DNA extraction and PCR amplification, specifically designed for genotyping applications. The kit combines a non-toxic DNA extraction solution with a ready-to-use Taq DNA Polymerase 2x Master Mix RED, enabling streamlined workflows from sample to PCR.
DNA extraction is completed in just 8 minutes using a simple two-step heating protocol, producing PCR-ready DNA without the need for purification, centrifugation, or dilution. The extracted DNA can be used directly for PCR and real-time PCR, significantly reducing processing time compared to conventional methods.
Key Features
- Rapid 8-minute DNA extraction with minimal handling
- PCR-ready DNA with no purification or centrifugation required
- Direct gel loading using RED master mix
- Suitable for genotyping across multiple sample types
- Scalable and automation-friendly workflow
- Non-toxic extraction chemistry
- Complete workflow from extraction to amplification in <1.5 hours
Kit Contents
- Q-Extract DNA Extraction Solution
- 5 × 10 mL
- Optimised for rapid DNA extraction
- Taq DNA Polymerase 2x Master Mix RED (1.5 mM MgCl₂)
- 5 × 1.25 mL
- Ready-to-use PCR master mix
Workflow Summary
- Sample lysis using Q-Extract solution (two-step heating)
- Obtain PCR-ready DNA extract
- Add master mix, primers, and water
- Perform PCR
- Load directly onto gel (no loading dye required)
Workflow Summary
Suitable for DNA extraction from:
- Mammalian tissues (mouse tails, ear snips, liver, kidney, lung)
- Plant leaves
- Saliva
- Bacteria
- Fish fins*
- Bird feathers*
*May require extended incubation for optimal lysis
Master Mix Composition
- Tris-HCl (pH 8.5)
- (NH₄)₂SO₄
- MgCl₂
- dNTPs (0.4 mM each)
- Taq DNA Polymerase
- Tween® 20
- Inert red dye and stabiliser
Performance Features
- Red dye allows direct loading onto agarose gel
- Dye front migrates at ~1000–2000 bp (0.5–1.5% agarose gel)
- Enables visual confirmation of pipetting and mixing
- No need for separate loading dye
Storage & Stability
Q-Extract DNA Extraction Solution:
- Long-term storage: -20 °C
- Short-term storage: +4 °C (up to 3 months)
- Stable through up to 20 freeze-thaw cycles
Taq DNA Polymerase 2x Master Mix RED:
- Long-term storage: -20 °C
- Short-term storage: +4 °C (up to 6 months)
Extracted DNA Stability:
- -20 °C: up to 1 week
- -80 °C: long-term storage
Quality Control
- Each batch of Q-Extract solution is functionally tested
- Taq DNA Polymerase tested for:
- No endonuclease activity
- No exonuclease activity
- No nicking activity
Typical Applications
- Genotyping and screening
- PCR amplification
- Real-time PCR (qPCR)
- Molecular biology research
- High-throughput workflows
Storage & Stability
- Significantly faster than traditional extraction methods
- Minimal hands-on time and simplified workflow
- Reduces risk of contamination and handling errors
- Suitable for a wide range of sample types
- Ideal for routine and high-throughput genotyping
Frequently Asked Questions (FAQs)
Q: What is the Q-Extract DNA Extraction PCR Kit used for?
A: The Q-Extract DNA Extraction PCR Kit is used for rapid DNA extraction and PCR amplification, particularly for genotyping and molecular biology applications.
Q: How fast is the DNA extraction process?
A: DNA extraction can be completed in approximately 8 minutes using a simple two-step heating protocol.
Q: Is DNA purification required before PCR?
A: No. The kit produces PCR-ready DNA, eliminating the need for purification, centrifugation, or dilution steps.
Q: What is the total workflow time from extraction to PCR?
A: The complete workflow from DNA extraction to PCR results can be completed in less than 1.5 hours.
Q: What sample types can be used with this kit?
A: The kit is compatible with a wide range of samples, including mammalian tissues, plant leaves, saliva, bacteria, fish fins, and bird feathers.
Q: How many reactions are included in the kit?
A: The kit provides reagents for up to 500 reactions, making it suitable for high-throughput applications.
Q: What is included in the kit?
A: The kit includes Q-Extract DNA Extraction Solution and Taq DNA Polymerase 2× Master Mix RED (1.5 mM MgCl₂), providing everything needed for extraction and PCR.
Q: Can PCR products be loaded directly onto a gel?
A: Yes. The included RED master mix contains a loading dye, allowing direct gel loading without additional preparation.
Q: What is the reaction volume for PCR?
A: The standard PCR reaction volume is 25 µL.
Q: Is the kit suitable for real-time PCR (qPCR)?
A: Yes. The extracted DNA is suitable for both standard PCR and real-time PCR (qPCR) applications.
Q: What are the storage requirements for the kit?
A: Both the extraction solution and master mix should be stored at -20 °C for long-term stability, with short-term storage possible at +4 °C for limited periods.
Q: How stable is the extracted DNA?
A: Extracted DNA can be stored at -20 °C for up to 1 week or at -80 °C for long-term storage.
Q: What are the advantages of using this kit?
A: Key advantages include rapid extraction, no purification steps, reduced hands-on time, direct gel loading, and high reproducibility.
Q: Is the kit suitable for high-throughput workflows?
A: Yes. The streamlined protocol and large number of reactions make it ideal for high-throughput genotyping and screening applications.
Q: Does the kit reduce the risk of contamination?
A: Yes. The simplified workflow with fewer handling steps helps reduce contamination risk and improve consistency.
Q: Is this kit intended for diagnostic use?
A: No. This product is for research use only and is not intended for diagnostic procedures.
| Brand | Ampliqon |
|---|---|
| Reaction Size | 500 Reactions |
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