DNA Loading Buffer, Blue, 5x1ml
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- Pack size: 5 x 1 ml
- Format: 5x concentrated solution
- Tracking dye: Bromophenol Blue
- Dye colour: Blue
- Dye front (1% agarose gel): 300 – 500 bp*
- Compatible gel types: Agarose gels, SDS DNA gels
- Storage: +4 °C for up to 6 months | -20 °C for longer term storage
- For Research Use Only. Not for use in diagnostic procedures.
- Other product sizes, combinations and customised solutions are available.
Kit Components - 5x Loading Buffer Blue
- Ficoll 400: 15 %, Tris-HCl pH 8.0: 10 mM, EDTA: 50 mM, Bromophenol Blue: 0.03 %
* Dye front position is dependent on agarose type, gel percentage, and buffer used. Value shown is for a 1% agarose gel.
Need a customised solution?
Optional accessories are available for purchase separately. If your order needs a customised configuration, our technical specialists will follow up with full details before your order is finalised. Contact us via the enquiry form or sales@lasec.com.
Installation, training and special delivery
Some products require installation, training, or special delivery (such as tail-lift trucks, extra labour, or hazardous material packaging). If your order needs this, we'll contact you with the additional cost before dispatch, so your final invoice may differ from the listed price.
Ordering from outside South Africa
Online ordering is currently limited to South African customers, but our full range is still available. Log an enquiry or email international@lasec.com and our international sales team will assist you through our traditional sales channels.
DISCLAIMER: All product images are for illustrative purposes only. Actual products may vary slightly in colours, controls, and visible features.
Availability:On Request
SKU
BAMPA608204
BrandAmpliqon
Product Overview
Ampliqon DNA Loading Buffers are 5x solutions formulated for loading DNA samples onto agarose or SDS DNA gels for gel electrophoresis. Each buffer contains Ficoll, Tris-buffer, and EDTA combined with one of four tracking dyes. The four-variant range allows laboratories to select the buffer most suited to the DNA fragment sizes under analysis.
Three Functional Roles
- Density: The DNA Loading buffers increase the density of the DNA sample, so it sinks to the bottom of the well. Ficoll serves as the density agent in this formulation.
- Visibility: The coloured tracking dye adds visibility to the DNA sample and provides a visual check that the sample has loaded correctly into the well.
- Migration monitoring: The coloured tracking dye also allows the operator to monitor the DNA migration progress during electrophoresis. Each dye migrates at a characteristic size.
Tracking Dye Variants
Cresol Red, Bromophenol Blue, Orange G, Xylene Cyanol FF
Preparation Protocol
For a 10 µl final loading volume: combine 2 µl of 5x Loading Buffer with 8 µl of DNA sample. Mix well and load onto the gel.
Storage and Stability
- -20 °C: Long-term storage. Expiry date stated on product label.
- +4 °C: Short-term option. Stable for up to 6 months.
Selecting the Correct Variant
The tracking dye choice depends on the size of the DNA fragments being analysed.
The front of the tracking dye should not run at the size of the DNA fragments because, especially dark dyes, obscure the DNA bands.
It is preferable to select a tracking dye that runs ahead of the DNA fragments being analysed.
| Brand | Ampliqon |
|---|---|
| Colour | Blue |
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