Total RNA Purification Kits, 100 Preps
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Format: Spin Column
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Size: 100 preps
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Total RNA Purification Kits for the rapid purification of total RNA, including microRNA, without the use of phenol
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Fast and easy processing using rapid spin-column format
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Isolate total RNA, from large rRNA down to microRNA (miRNA)
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No phenol or chloroform extractions
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Isolate high-quality total RNA from a variety of sources
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RNA can be isolated and detected from as little as a single animal cell
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For research use only and NOT intended for in vitro diagnostics.
Optional accessories are available for purchase separately. If you require a customised solution, please contact our technical specialists by selecting the "Add to enquiry" button and using our enquiry form or at sales@lasec.com.
Installation, training and special delivery requirements, such as the need for tail-lift trucks and additional labour and installation for large equipment and hazardous material (Hazmat) packaging, are chargeable in addition to standard freight and will be quoted separately before dispatch.
Online ordering is currently limited to South African customers, but our full range is still available. If you are ordering from outside South Africa, please log an enquiry or email internationallabsales@lasec.com, and our international sales team will gladly assist you through our traditional sales channels.
Availability:On Request
SKU
BNOR37500
BrandNorgen Biotek Corp.
These Total RNA Purification Kits are suitable for the isolation of total RNA from a range of samples, including cells, bacteria, yeast, viruses, and bodily fluids, including plasma/serum, blood, saliva, CSF, and more. Extract high-quality and pure RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more. These kits purify all sizes of RNA, from large mRNA and lncRNA down to microRNA (miRNA), in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate, irrespective of size. Moreover, when the RNA sequences are small (e.g., miRNA), the column binds small RNAs regardless of their GC content.
Advantages of Total RNA Purification Kits:
- Extract high-quality and quantity total RNA, including miRNA
- No phenol step required; isolate all RNA in one fraction
- Bind and elute all RNA, irrespective of size or GC content, without bias
- Very sensitive and linear down to a few cells without the need for carrier RNA
- Isolate from a wide variety of specimens
- Purified RNA is suitable for a variety of downstream applications, including small-RNA sequencing.
- Available in a variety of formats to suit your needs
- Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
Total RNA Purification Kit Components, 100 Preps:
- Buffer RL: 2 x 40 mL
- Wash Solution A: 2 x 38 mL
- Elution Solution A: 2 x 6 mL
- Mini Spin Columns: 100
- Collection Tubes: 100
- Elution tubes (1.7 mL): 100
- Product Insert: 1
| Kit Specifications | |
| Maximum Binding Capacity | Up to 50 μg RNA |
| Maximum Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material | |
| Animal Cells | 3 x 106 cells |
| Animal Tissues | 10 mg (for most tissues*) |
| Blood | 100 μL |
| Plasma/Serum | 200 μL |
| Bacteria | 1 x 109 cells |
| Yeast | 1 x 108 cells |
| Fungi | 50 mg |
| Plant Tissues | 50 mg |
| Time to Complete 10 Purifications | 20 minutes |
| Average Yield | |
| HeLa Cells (1 x 106 cells) | 15 μg |
| E. coli (1 x 109 cells) | 50 μg |
| Brand | Norgen Biotek Corp. |
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